Koushafar, Z. & A. A. Mohammadpour, 2019. Morphological study of the midbrain tectum in ostrich (Struthio camelus) embryo. Bulg. J. Vet. Med., 22, No 2,[143][144][145][146][147][148][149][150][151] In this study the morphological features of the optic tectum in ostrich embryo were studied macroscopically and microscopically. After gross anatomical study, fixed specimens of the optic lobes in 30 th , 36 th and 40 th embryonic days were processed for paraffin sections. Sections were stained by Harris haematoxylin and eosin (H&E), Luxol Fast Blue/Cresyl Echt Violet and Malory PTAH dyes. The optic lobes had large volumes even on the 30 th embryonic day and increased proportionally to age. The optic lobe consisted of two parts: gray matter (outer) and white matter (inner). The first external layer of the optic lobe e.g. molecular layer consisted of neural fibres, neuroglia and scarce small neurons. The most common appearance of the optic lobes was characterised by small to medium-sized neurons (rounded to pyramid-shaped with large and pale nucleus consistong of obvious nucleoli arranged in three layers whose thickness increased in the deeper one) supported by neuroglia. Larger size neurons and occasionally multipolar neurons were presented in the interior compared with these layers. The lateral mesencephalic nucleus was detectable in the optic lobe base even on 30 th embryonic day and was composed of few multipolar neurons supported by neuroglia. The tectal ventricles were lined with simple cuboidal ciliated ependymal cells in the embryonic period. As embryonic age increased, the ratio of tectal ventricle volume to its thickness decreased. Special stainings showed that Nissl bodies and myelin fibres, also glial fibres were available from the 30 th embryonic day and that their density, especially myelin fibres density, increased with age.
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