Background: Telomeres are evolutionary, specialized terminal structures at the ends of eukaryotic chromosomes containing TTAGGG repeats in human. Several human diseases have been known to be associated with dramatic changes in telomere length. The aim of the present study was to assess the correlation between the relative leukocyte telomere length (LTL) and infertility in a group of Iranian azoospermic males. Methods: In this casecontrol pilot study, relative telomere length (RTL) of peripheral blood leukocytes from a total of 30 idiopathic nonobstructive azoospermic males and 30 healthy fertile males was evaluated using real-time PCR. RTL was calculated as T (telomere)/S (single copy gene) ratio and compared between infertile and fertile groups. Results: Patients with azoospermia showed significantly shorter RTL than fertile males (0.54 vs. 0.84, p < 0.05). The area under the receiver operating characteristic (ROC) curve was estimated to be 99.8%, suggesting LTL as a potential marker for the diagnosis of azoospermia. Conclusion: Our findings demonstrated a probable association between telomere shortening and azoospermia in a population of Iranian infertile men affected by idiopathic azoospermia.
Aim:
Different studies have been performed to investigate the stem cell administration as promising tool for recovery of injured tissue in Multiple Sclerosis (MS), the most common demyelinating disease.
Methods:
In the present systematic review, the electronic databases of PubMed and ScienceDirect were searched to screen English language studies published until April 2020.
Results:
The results obtained from experimental autoimmune encephalomyelitis (EAE) animals revealed that modified mesenchymal stem cells (MSCs) transplantation was associated with remyelination, inflammation suppression and oligodendrocyte precursor cells regeneration. Clinical trials indicated that 70% of the patients with MS showed disease stabilization following MSCs administration.
Conclusion:
Although MSC therapy has showed to be effective in the improvement of some patients with MS, designing of larger placebo-controlled clinical trials with MSCs expressing immune-regulators or MSCs-exosomes may provide the novel viewpoint in the treatment of MS.
Prostate cancer (PCa) was known as the second most common cancer in men. Although there were several approaches to treat this disease, the cost and side effects of some approaches have encountered the patients with challenges. Therefore, the design of new therapy methods could be useful in the management of this disease. For this purpose, the synergic effect of Epigallocatechin gallate (EGCG) and conditioned medium derived from Wharton's jelly mesenchymal stem cells (WJ-MSCs) were studied on prostate cancer LNCaP cells. In this study, LNCaP cells were treated with different concentrations of EGCG and conditioned medium derived from WJ-MSCs (WJCM). The viability of treated cells was determined by using cell proliferation assay. Then, the expression of androgen receptor (AR and PSA) and apoptotic (BAX, CASP3 and CASP7) pathway genes were defined by Real time PCR. The analysis of the data indicated that the treatment with 400µM EGCG in combination with 50% WJCM (0% FBS) for 72 hours decreased expression of AR and PSA genes as well as the enhanced expression of BAX, CASP3 and CASP7 genes in the LNCaP cells (p<0.05). The obtained results suggested that the combination therapy of EGCG and WJCM had an anticancer effect on LNCaP cells through activation of apoptotic pathway and suppression of androgen receptor pathway.
Prostate cancer (PCa) was known as the second most common cancer in men. Although there were several approaches to treat this disease, the cost and side effects of some approaches have encountered the patients with challenges. Therefore, the design of new therapy methods could be useful in the management of this disease. For this purpose, the synergic effect of Epigallocatechin gallate (EGCG) and conditioned medium derived from Wharton's jelly mesenchymal stem cells (WJ-MSCs) were studied on prostate cancer LNCaP cells. In this study, LNCaP cells were treated with different concentrations of EGCG and conditioned medium derived from WJ-MSCs (WJCM). The viability of treated cells was determined by using MTT assay. Then, the expression of androgen receptor (AR and PSA) and apoptotic (BAX, CASP3 and CASP7) pathway genes were defined by Real time PCR. The analysis of the data indicated that the treatment with 400µM EGCG in combination with 50% WJCM (0% FBS) for 72 hours decreased expression of AR and PSA genes as well as the enhanced expression of BAX, CASP3 and CASP7 genes in the LNCaP cells (p < 0.05). The obtained results suggested that the combination therapy of EGCG and WJCM had an anticancer effect on LNCaP cells through activation of apoptotic pathway and suppression of androgen receptor pathway.
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