Objective The aim of the study was to evaluate the association between subgingival restorations and the target periodontopathogenic bacteria (Pg, Td and Pi) in subgingival biofilm during one year after combined restorative-periodontal treatment.Material and Methods Seventeen systemically healthy subjects, who were positive for the presence of three cervical lesions associated with gingival recessions in three different adjacent teeth, were included in the study. A total of 51 combined defects were treated with connective tissue graft plus a nanofilled composite resin (NCR+CTG), a resin-modified glass ionemer cement (RMGI+CTG) and a fluoride-releasing resin material with pre-reacted glass (PRG), called giomer (Giomer+CTG). Periodontal clinical measurements and subgingival plaque samples were obtained from all combined defects at baseline and at 6 and 12 months after the surgery. The number of bacteria were evaluated by the real-time polymerase chain reaction (qPCR) method.Results No statistically significant difference in the amount of DNA copies of Pg, Td and Pi was observed in any of the groups at any time points (p>0.05). In addition, there was no statistically significant difference in the amount of DNA copies of the bacteria at baseline and at 6 and 12 months postoperatively, regardless of treatment group (p>0.05).Conclusion This study suggests that subgingivally placed NCR, RMGI and giomer restorations can show similar effects on periodontopathogenic bacteria in the treatment of gingival recessions that are associated with noncarious cervical lesions (NCCLs).
This study was carried out to determine the effects of storage duration on nutrient composition and silage quality parameters. Corn was used as silage material. Corn (31.41% dry matter) was harvested at the dough stage and fermented for 90, 104, 118, 132, 146, 160, 174, 188 and 202 days in three trench silos. The samples were brought to laboratory every 14 days. This process was repeated 9 times. After the 132th day, whereas silage crude protein (CP), ether extract (EE), ash and crude fiber (CF) contents decreased, nitrogen free extract (NFE) content increased. Lactic acid concentration of corn silage increased until the 118th day but decreased between the 118th and the 160th days. On the contrary, of decrease in LA concentration, acetic acid concentration increased depending on storage time. Ammonia nitrogen and CO2 concentration of silage increased decreased with progressing time. Storage time had significant influence on Flieg scores. The lowest score was found between days the 104th-118th. In this research, it was observed that there was a change in silage nutrient contents and fermentation characteristics with increasing storage time.
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