The purpose of this study was to quantify the microbial load and to detect potentially pathogenic germs on the surface of absorbent food pads in the packaging of fresh chicken and minced meat marketed in the retail units in Romania. The load in E. coli, as an indicator of faecal contamination, was significantly lower than that of Enterobacteriacea and coliform germs. Congo red-binding E. coli strains have been identified. The affinity for the Congo red dye is considered a phenotypic marker for the presence of curli fibers. These surface formations give the bacterial strains the ability to form biofilm on various surfaces. Their presence also correlates with the property of these bacterial strains to trigger severe infections in humans. These strains showed 100% resistance to fourth-generation cephalosporins, 87.5% to third-generation cephalosporins and 75% to quinolones. A single strain was resistant to meropenem. All the tested strains were susceptible to gentamicin. Coagulase-positive staphylococci were identified in 40% of the analyzed samples. Of the 30 strains of Staphylococcus spp. tested (coagulase-positive and negative), 63.3% proved to be methicillin-resistant and 43.3% had resistance to sulfamethoxazole-trimethoprim. In one sample, it was detected Salmonella infantis. Thus, it can be appreciated that these absorbent food pads may be a source of direct contamination with potentially pathogenic and / or antibiotic-resistant germs for the persons who manipulates them in their domestic environment, as well as a source of cross-contamination of food and other surfaces in the kitchen.
The infection with Salmonella in pigs farms is usually endemic and largely asymptomatic. The present study aimed the importance of feeding type (dry/wet) administered in the transmission of Salmonella in pigs farms. We had studied two farms, one in which pigs received wet feeding and at the other the given ration was dry. From each farm were collected 100 fecal and 50 feed samples. After analyzing the samples collected, it was found that the number of positive samples on the farm were the ration was liquid, was 34% from the feces and (2%) from the feed, which was reduced in comparison with the farm were the ration was dry, where the positive samples were (61%) for feces, and 4% from feed. The results support the ideas that liquid feeding ensures less chance of contamination by 27% compared to dry feeding, because is inducing an increases of organic acids which facilitates normal bacterial growth, leading to reduction of Salmonella.
The aim of the present study was to monitor the carrier status for Salmonella spp. in piglets from two farms (farm A and farm B), collecting samples from piglets after weaning until fattening, on the same animals. For this purpose 60 faecal samples were collected immediately after weaning until tansfer to fattening facilities. By analyzing faecal samples collected from both farms (farm A and B), a 50% Salmonella spp. load was observed in weaned piglets, and an increase by 7% to young stock. Identification and isolation of Salmonella was carried out by EN ISO 6579: 2003 method followed by serum agglutination, and to isolate Salmonella spp. serovars API 20E method was used.
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