αs1-, αs2-, β- and κ-caseins
from Somali camels (Camelus dromedarius) were
purified by acid precipitation at pH 4·4, crudely separated into
an α-CN and a β-CN
fraction and further purified by reversed-phase HPLC. Fragments of tryptic
digests
were sequenced. Amino acid patterns obtained were used to screen a cDNA
library
constructed from mRNA from lactating udder tissue. Full length clones corresponding
to the four caseins were sequenced. The numbers of residues in the
sequences deduced were αs1-CN 207, αs2-CN
178,
β-CN 217, κ-CN 162. Percentage
similarity to bovine proteins was αs1-CN A 39,
αs2-CN 56, β-CN 64, κ-CN 56.
Acid-precipitated casein of pooled milk was separated by reversed-phase
HPLC and
monitored at 220 nm, and its composition, estimated from peak integration,
was
(g/kg total casein) αs1-CN 220, αs2-CN
95,
β-CN 650, κ-CN 35. Degrees of
phosphorylation and glycosylation were determined by laser ionization mass
spectrometry and sequence pattern analysis. Molecular masses determined
were
(kDa) αs1-CN A, 24·755 and 24·668; αs1-CN
B,
25·293; αs2-CN 21·993; β-CN, 24·900;
κ-CN 22·294–22·987. The pH values of the most
probable isoelectric points were: αs1-CN A 6P 4·41,
αs1-CN B 6P 4·40, αs2-CN 9P 4·58,
β-CN 4P 4·66, κ-CN 1P, with ten sialic acid residues
bound, 4·10.
Introduction Present distribution and milk production Main components Gross composition Overall protein composition Casein fractions Size distribution of casein Whey protein fractions Lipids Fatty acid composition CONTENTS PAGE 603 604 605 605 606 607 609 611 612 612 Phospholipids Physical state and properties of fat globules Lactose Minerals and vitamins Enzymic coagulation Effects of heat on milk Antibacterial activity Conclusions References INTRODUCTION PAGE 613 614 616 616 617 620 623 623 624 Camels belong to the family Camelidae and thereby to the suborder Tylopoda. The tylopoda themselves belong to the order Artiodactyla or cloven-footed animals. The family of Camelidae contains the genera Camelus (old world camel) and Lama (new world camel). The Camelidae originated in North America where the earliest fossil remains of Camelidae have been found. The genus Camelus migrated from North America in the late Tertiary across the then existing land bridge to Asia and Africa. The llamas on the other hand reached South America in the ice age across the Central American land bridge. Included in the genus Camelus are the one-humped dromedary (Camelus dromedarius) and the two-humped bactrian (Camelus bactrianus). The term dromedary is derived from the Greek 'dromados' (run) and in the strict sense is used for riding camels. The name 'Bactrian' for the two-humped camel refers to the area ' Baktria' in North Afghanistan where this type of camel is thought to have originated. The dromedary is slim, long-legged, short-haired and has its habitat in warm arid and semi-arid areas. The bactrian is stockier, short-legged and has a thicker and longer coat than the dromedary. It mainly occurs in cold and mountainous areas (Simpson, 1945; Zeuner, 1963). This review is concerned entirely with the one-humped camel; the term ' camel' should therefore be taken to refer to Camelus dromedarius unless specifically stated otherwise. The majority of the studies conducted on camels concentrate on anatomical features, traditional management and physiological adaptation to desert conditions (Cauvet,
Yam starch of the species Dioscorea alata and D. cayenensis‐rotundata was isolated, characterized by differential scanning calorimetry (DSC), X‐ray diffraction, iodine titration, hot‐stage microscopy and rheology, and compared to potato (Solanum tuberosum) and tapioca (Manihot esculenta) starch. The amylose content of yam starch was around 24% and tended to decrease during postharvest storage. Wide‐angle X‐ray diffraction showed a mixed A‐ and B‐type crystalline packing. Differences between yam starches from different species and varieties were revealed by DSC. However, the largest differences between the different yam starches were found in terms of their rheological behavior. Yam starch shows an intermediate viscosity increase upon initial heating, but a stable viscosity during further thermal treatment in the Viscograph. Hot‐stage microscopy showed a good preservation of the granular yam structure during heating, which is thought to be responsible for a rather high viscosity at low stress paired with a strong shear‐thinning effect. The mechanical properties of yam starch gels suggest that the preservation of the granular integrity upon swelling limits amylose leaching, but that the aging behavior is primarily determined by the short‐term changes of the amylose fraction. Yam starch has potential as thickening and gelling agent in food.
The peptidoglycan recognition protein, PGRP, known as an intracellular component of neutrophils, has been isolated from camel (Camelus dromedarius) milk by acid precipitation followed by heparin-sepharose affinity chromatography of the supernatant. The mean concentration in milk was about 120 mg/L. It decreased during lactation by 19% and increased in the event of severe mastitis by 45%. The protein bound to lactic acid bacteria and other gram-positive bacteria with an affinity similar to that reported for the human and murine orthologs, although the isoelectric point of the molecule was distinctly higher at pH 9.02. The N-terminus of mature camel PGRP was determined as NH2-ArgGluAspProPro-CO2H. Calculated and measured molecular masses were both 19.1 kDa, excluding the possibility of posttranslational modifcation or binding of cation ligands. The peptide probably builds a homotrimer at high concentration. The corresponding mRNA was isolated from lactating mammary gland tissue, and 5.3 kbp of the corresponding gene was sequenced. Similarities were found to the camel lactoferrin gene with regard to sites of expression and to the region 5' upstream to the gene.
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