BackgroundDue to the increasing demands of energy and depletion of fossil fuel, bamboo is considered to be one of the most important renewable biological resources on the basis of its advantages of rapid growth ability and rich reserves. Cellulose, hemicellulose, and lignin are the three most important constituents in moso bamboo. Their concentrations and, especially, their microscopic distributions greatly affect their utilization efficiency and other physical properties as a biomass resource. However, no studies have achieved a quantitative visualization of the distribution of lignocellulose concentrations in transverse sections of bamboo. Therefore, this study proposed the use of quantitative multivariate spectral analysis to reveal the micro-chemical distribution of lignocelluloses in bamboo based on an integration of FTIR macro- and micro-spectroscopic imaging techniques.ResultsMultivariate calibration models for the quantitative determination of lignocelluloses of bamboo were developed based on FTIR macro-spectroscopy, and the quantitative calibration models based on the FTIR characteristic bands showed an excellent performance with determination coefficients of 0.933, 0.878, and 0.912 for cellulose, hemicellulose, and lignin, respectively. These quantitative models were then utilized to the FTIR micro-spectroscopy of bamboo transverse sections which were corrected using a direct standardization algorithm. Subsequently, the micro-chemical distributions of cellulose, hemicellulose, and lignin were obtained based on the integration of the multivariate calibration models and corrected FTIR micro-spectroscopy. The combination of the multivariate calibration models and calibration transfer algorithm resulted in a final quantitative visualization of the chemical distributions of lignocelluloses in moso bamboos.ConclusionsIntegration of the FTIR macro- and micro-spectroscopic imaging techniques can provide comprehensive information that can be used to exploit the resource of moso bamboo to develop biofuels and biosynthetic materials.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.