2'-O-sulfated glycoside and a 2'-O-linked glucuronic acid containing disaccharide, were detected in the red beet culture.
ACKNOWLEDGEMENTSThe author thanks Profs N Amrhein and W Barz for providing the red beet and soybean cell cultures, respectively. Abstract: Nitri®cation inhibitory activity of halomethyl-1,3,5-triazines was determined by measuring the inhibitory activities on ammonia-oxidation to nitrate (NO 3 À -N) in an upland soil and on ammonia-oxidation to nitrite (NO 2 À -N) by Nitrosomonas europaea ATCC 25978 (ATCC) and Nitrosomonas sp TK 794 (TK).
REFERENCESWithin the chlorinated trimethyl-1,3,5-triazines, those bearing at least one trichloromethyl group inhibited nitri®cation more strongly, both in soil and in cell suspension of ATCC, than other mono-or dichlorinated methyl-1,3,5-triazines. Introduction of an amino group to 2,4,6-tris(trichloromethyl)-1,3,5-triazine gave 10-and 100-fold increases of nitri®cation inhibitory activity in soil and ATCC cell culture, respectively. Within the trihalomethyl-1,3,5-triazines, those having tribromomethyl group(s) exhibited rather weaker nitri®cation inhibition in soil than the corresponding trichloromethyl-1,3,5-triazines, although they showed a strong inhibition in cell suspension.Ammonium oxidation in ATCC was inhibited more strongly than that in TK. In QSAR studies, the optimum log P values were calculated as c 4.30. By using this value it will become possible to design highly active trichloromethyl-1,3,5-triazine nitri®-cation inhibitors.