Aim: To examine the immunomodulatory effect of exosomes originating from gingival mesenchymal stem cells (GMSC-Exo) on periodontal bone regeneration and its role in the regulation of the nuclear-factor kappaB (NF-κB) and Wnt/β-catenin pathways in the periodontal inflammatory microenvironment. Materials and Methods: First, periodontal ligament stem cells (PDLSCs) were treated with GMSC-Exo or Porphyromonas gingivalis-derived lipopolysaccharide (P.g-LPS) in vitro. Quantitative real-time PCR (qRT-PCR) and western blot were carried out to detect the expressions of osteogenic differentiation-related factors in cells. Further, PDLSCs were treated with P.g-LPS or inhibitors. The expression of NF-κB pathwayrelated factors as well as of Wnt/β-catenin pathway-related factors were detected by qRT-PCR and western blot.Results: GMSC-Exo treatment promoted the expression of osteogenic differentiationrelated factors within PDLSCs in both normal and inflammatory environments. Further investigations showed that GMSC-Exo could also inhibit the P.g-LPS-induced activation of the NF-κB pathway, leading to the up-regulation of the Wnt/β-catenin pathway.When the Wnt/β-catenin signalling was blocked, the inhibitory effect of GMSC-Exo on the NF-κB pathway was abolished.Conclusions: GMSC-Exo could promote the osteogenic differentiation of PDLSCs.There could be mutually exclusive regulatory roles between the NF-κB and Wnt/βcatenin signalling pathways in a periodontal inflammatory environment. GMSC-Exo exhibited an effective cross-regulation ability for both pathways.
A novel flame retardant containing phosphorus and nitrogen elements, ammonium salt of N,Ndimethylene p-benzenesulfonic phosphonic acid (ADBSPA), was prepared by one-step continuous chemical method. The chemical structure of the ADBSPA was determined by nuclear magnetic resonance spectrum (NMR) and Fourier transform infrared (FTIR), and ADBSPA was processed onto cotton materials by immersion method. The flame retardancy of coated cotton materials was analyzed by limiting oxygen index (LOI) and Vertical burning test. The LOI value of cotton coated with 350 g/L ADBSPA increased from the original 18.2 ± 0.2% to 31.7 ± 0.2%, and the damaged length of this sample reduced to 6.6 ± 0.1 cm, which proved that ADBSPA enhanced the anti-burning ability of cotton materials. Cone calorimetry was used to evaluate the combustion behavior of cotton materials before and after coating. Compared with original sample, ADBSPA changed the combustion path of cotton materials, and the intuitive performance was that the ADBSPA-cotton-4 reserved 25.1 ± 0.5% of char residue at 250 s, and its peak heat release rate (PHRR) greatly reduced to 17 ± 1 kW/m 2 . The surface morphology of uncoated and coated cotton materials was characterized by scanning electron microscopy (SEM). It was observed that the coated sample still maintained a complete fiber skeleton after burning. In addition, thermogravimetric (TG) analysis confirmed that the coated cotton materials had good thermo-oxidative and thermal stability. All results pointed out that the phosphorus and nitrogen elements contained in ADBSPA as well as cotton cellulose constituted the three basic conditions of intumescent flame-retardant system. The polyphosphoric acid compounds formed by phosphorus element at high temperature promoted the dehydration of cotton fibers into char layer. The non-flammable gases produced by the nitrogen element in the combustion process covered the surface of fabrics, thereby playing the role of shielding heat and diluting combustible gases.
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