Whereas prospects of bioremediation
for a vanadium(V) [V(V)]-contaminated
environment are widely recognized, reported functional species are
extremely limited, with the vast majority of Gram-negative bacteria
in Proteobacteria. Herein, the effectiveness of V(V) reduction is
proved for the first time by Lactococcus raffinolactis, a Gram-positive bacterium in Firmicutes. The V(V) removal efficiency
was 86.5 ± 2.17% during 10-d operation, with an average removal
rate of 4.32 ± 0.28 mg/L·d in a citrate-fed system correspondingly.
V(V) was bio-reduced to insoluble vanadium(IV) and distributed both
inside and outside the cells. Nitrite reductase encoded by gene nirS mainly catalyzed intracellular V(V) reduction, revealing
a previously unrecognized pathway. Oxidative stress induced by reactive
oxygen species from dissimilatory V(V) reduction was alleviated through
strengthened superoxide dismutase and catalase activities. Extracellular
polymeric substances with chemically reactive hydroxyl (−OH)
and carboxyl (−COO–) groups also contributed
to V(V) binding and reduction as well as ROS scavenging. This study
can improve the understanding of Gram-positive bacteria for V(V) bio-detoxification
and offer microbial resources for bioremediation of a V(V)-polluted
environment.
Aquifer co-contamination by vanadium (V) and pentachlorophenol (PCP) involves complicated biogeochemical processes that remain poorly understood, particularly from the perspective of microbial metabolism. Batch experiment results demonstrated that V(V) and PCP could be competitively bio-reduced, with 96.0 ± 1.8% of V(V) and 43.4 ± 4.6% of PCP removed during 7 d operation. V(V) was bio-transformed to vanadium (IV), which could precipitate naturally under circumneutral conditions, facilitating the removal of up to 78.2 ± 3.1% dissolved total V. The PCP reductive dechlorination products were mainly 2,4,6-trichlorophenol and 4-monochlorophenol with lower toxicity. High-throughput 16S rRNA gene sequencing indicated that Pseudomonas, Soehngenia, and Anaerolinea might be responsible for the two bio-transformations, with detected functional genes of nirS and cprA. Extracellular reduction by cytochrome c and intracellular conversion by nicotinamide adenine dinucleotide (NADH) occurred for both V(V) and PCP. Extracellular proteins in microbial-secreted extracellular polymeric substances (EPS) might also be involved in these enzymatic bioprocesses. EPS could protect microbial cells through V(V) binding by the chemically reactive carboxyl (COO-), and hydroxyl (-OH) groups. These findings elucidate the metabolic processes during anaerobic V(V) and PCP biotransformation, advance understanding of their biogeochemical fates, and provide a foundation on which to develop novel strategies for remediation of co-contaminated aquifers.
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