YUTAKA MAKIMOTO, Ell TAKAHASHI, IDROSHI TAKASUGI Tokyo research laboratory, Kyowa Hakko Kogyo co., Tokyo, Japan Microcarrier (M.c.) for the large-scale culture of anchorage dependent animal cells is available. We have established a micorcarrier perfusion culture (llrscale) of recombinant CHO cells to produce humanized monoclonal antibody, KM8400 and evaluated the productivity of this culture. A cell density of2.5x10 7 cells/ml was reached on day 13 and this culture maintained for 26 days. The total amount ofKM8400 produced was 3.5g.During the culture's stationary phase, specific production rate was 1.5 fold higher than in the growth phase. Surprisingly, we found that the productivity of this cell line increased with the increase of G1 phase cell population.
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