As facial color pattern around the eyes has been suggested to serve various adaptive functions related to the gaze signal, we compared the patterns among 25 canid species, focusing on the gaze signal, to estimate the function of facial color pattern in these species. The facial color patterns of the studied species could be categorized into the following three types based on contrast indices relating to the gaze signal: A-type (both pupil position in the eye outline and eye position in the face are clear), B-type (only the eye position is clear), and C-type (both the pupil and eye position are unclear). A-type faces with light-colored irises were observed in most studied species of the wolf-like clade and some of the red fox-like clade. A-type faces tended to be observed in species living in family groups all year-round, whereas B-type faces tended to be seen in solo/pair-living species. The duration of gazing behavior during which the facial gaze-signal is displayed to the other individual was longest in gray wolves with typical A-type faces, of intermediate length in fennec foxes with typical B-type faces, and shortest in bush dogs with typical C-type faces. These results suggest that the facial color pattern of canid species is related to their gaze communication and that canids with A-type faces, especially gray wolves, use the gaze signal in conspecific communication.

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Various training methods have been developed for animal husbandry and health care in zoos and one of these trainings is blood collection. One training method, recently widely used for blood collection in Ursidae, requires setting up a sleeve outside the cage and gives access to limited blood collection sites. A new voluntary blood collection method without a sleeve was applied to the Andean bear (Tremarctos ornatus) and Asiatic black bear (Ursus thibetanus) with access to various veins at the same time. The present study evaluated the effectiveness of this new method and suggests improvements. Two Andean and two Asiatic black bears in Yokohama and Nogeyama Zoological Gardens, respectively, were trained to hold a bamboo pipe outside their cages. We could, thereby, simultaneously access superficial dorsal veins, the dorsal venous network of the hand, the cephalic vein from the carpal joint, and an area approximately 10 cm proximal to the carpal joint. This allowed us to evaluate which vein was most suitable for blood collection. We found that the cephalic vein, approximately 10 cm proximal to the carpal joint, was the most suitable for blood collection. This new method requires little or no modification of zoo facilities and provides a useful alternative method for blood collection. It could be adapted for use in other clinical examinations such as ultrasound examination.

The ploughshare tortoise (Astrochelys yniphora) is an endangered species. It is important to increase the number of tortoises in the wild and captivity; however, breeding in captivity, especially outside of the natural habitat area, has been unsuccessful worldwide. As a result of an emergency rescue due to a violation of the Act of Conservation of Endangered Species of Wild Fauna and Flora, Nogeyama Zoological Gardens (Yokohama, Japan) has housed ploughshare tortoises, and a natural breeding trial started in 2011. In this case, the captive environment in Nogeyama, especially room temperature and humidity, imitated the environment in the natural habitat of northwest Madagascar. The maximum temperature during the day was 30.8–32.5°C throughout the year, but the minimum temperature was 24°C during December and January, representing the rainy season, and 17°C during July, representing the dry season. The humidity was adjusted and the room was not humidified between November and February (dry season) but was humidified by sprinkling water once every few days in March and daily between April and October (rainy season). Natural breeding and egg‐laying were observed 1 year after the captive environmental change. Three neonates were hatched from nine eggs in 3 years after the beginning of the breeding program. This study showed that for natural breeding of the ploughshare tortoises, it is important to adjust the temperature and humidity of the captive environment to imitate the temperature and humidity of the natural habitat.

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