Coffee is a complex mixture of many bioactive compounds possessing anti-inflammatory properties. However, the mechanisms by which coffee exerts anti-inflammatory effects remains unclear and the active ingredients have not yet been identified. In this study, we found that coffee extract at more than 2.5%(v/v) significantly inhibited LPS-induced inflammatory responses in RAW264.7 cells and that antiinflammatory activity of coffee required the roasting process. Interestingly, we identified pyrocatechol, a degradation product derived from chlorogenic acid during roasting, as the active ingredient exhibiting anti-inflammatory activity in coffee. HPLC analysis showed that 124 μM pyrocatechol was included in 100% (v/v) roasted coffee. A treatment with 5%(v/v) coffee extract and more than 2.5 μM pyrocatechol inhibited the LPS-induced activation of NF-κB and also significantly activated Nrf2, which acts as a negative regulator in LPS-induced inflammation. Furthermore, intake of 60% (v/v) coffee extract and 74.4 μM pyrocatechol, which is the concentration equal to contained in 60% (v/v) coffee, markedly inhibited the LPS-induced inflammatory responses in mice. Collectively, these results demonstrated that pyrocatechol, which was formed by the roasting of coffee green beans, is one of the ingredients contributing to the anti-inflammatory activity of coffee.
Difference-in-level detection in outdoor scenes has various possible applications, including walking assistance for blind people, robot walking assistance, and mapping the hazards of factory premises. It is difficult to detect all outdoor differences in level, such as RGB or RGB-D images, not only including road curbs, which are often targeted for detection in automated driving, but also differences in level on factory premises and sidewalks, because the pattern of outdoor differences in level is abundant and complex. This paper proposes a novel method for detecting differences in level from RGB-D images with segmented ground masks. First, image patches of differences in level were extracted from outdoor images to create the dataset. The change in the normal vector of the contour part on the detected plane is used to generate image patches of the difference in level, but this method strongly depends on the accuracy of planar detection, and it detects only some differences in level. Then, we created the dataset, consisting of image patches and including the extracted differences in level. The dataset is used for training a deep learning model for detecting differences in level in outdoor images without limitations. In addition, because the purpose of this paper is to detect differences in level in outdoor walking areas, regions in the image other than the target areas were excluded by the segmented ground mask. For the performance evaluation, we implemented our algorithm using a modern smartphone with a high-performance depth camera. To evaluate the effectiveness of the proposed method, the results from various inputs, such as RGB, depth, grayscale, normal, and combinations of them, were qualitatively and quantitatively evaluated, and Blender was used to generate synthetic test images for a quantitative evaluation of the difference in level. We confirm that the suggested method successfully detects various types of differences in level in outdoor images, even in complex scenes.
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