Approximate layer-averaged equations describing the mechanics of turbid underflows are derived. Closure of the equations describing the balance of fluid mass, sediment mass, and mean flow momentum provides for the delineation of a three-equation model. A description of sediment exchange with the bed allows for the possibility of a self-accelerating turbidity current in which sediment entrainment from the bed is linked to flow velocity. A consideration of the balance of the mean energy of the turbulence yields a constraint on physically realistic solutions to the three-equation model. It is shown that the self-acceleration predicted by the three-equation model is so strong that the energy constraint fails to be satisfied. In particular, the turbulent energy consumed in entraining new bed sediment exceeds the supply of energy to the turbulence, so that the turbulence, and thus the turbidity current, must die. The problem is rectified by the formulation of a four-equation model, in which an explicit accounting is made of the mean energy of the turbulence. Sediment entrainment from the bed is linked to the level of turbulence in the four-equation model. Self-acceleration is again predicted, although it is somewhat subdued compared with that predicted by the three-equation model. The predictions of both models are summarized over a wide range of conditions.
Soybeans (Glycine max (L.) Merr.) and certain other legumes excrete isoflavones from their roots, which participate in plantmicrobe interactions such as symbiosis and as a defense against infections by pathogens. In G. max, the release of free isoflavones from their conjugates, the latent forms, is mediated by an isoflavone conjugate-hydrolyzing -glucosidase. Here we report on the purification and cDNA cloning of this important -glucosidase from the roots of G. max seedlings as well as related phylogenetic and cellular localization studies. The purified enzyme, isoflavone conjugate-hydrolyzing -glucosidase from roots of G. max seedling (GmICHG), is a homodimeric glycoprotein with a subunit molecular mass of 58 kDa and is capable of directly hydrolyzing genistein 7-O-(6؆-O-malonyl--D-glucoside) to produce free genistein (k cat , 98 s ؊1 ; K m , 25 M at 30°C, pH 7.0). GmICHG cDNA was isolated based on the amino acid sequence of the purified enzyme. GmICHG cDNA was abundantly expressed in the roots of G. max seedlings but only negligibly in the hypocotyl and cotyledon. An immunocytochemical analysis using anti-GmICHG antibodies, along with green fluorescent protein imaging analyses of Arabidopsis cultured cells transformed by the GmICHG:GFP fusion gene, revealed that the enzyme is exclusively localized in the cell wall and intercellular space of seedling roots, particularly in the cell wall of root hairs. A phylogenetic analysis revealed that GmICHG is a member of glycoside hydrolase family 1 and can be co-clustered with many other leguminous -glucosidases, the majority of which may also be involved in flavonoid-mediated interactions of legumes with microbes.
ABSTRACT.Appropriate expressions describing the motion of powder-snow avalanches are derived. The model consists of four equations, i.e. the conservation equations of fluid mass, snow-particle mass, momentum of the cloud, and kinetic energy of the turbulence. Insofar as the density difference between the avalanche and the ambient air becomes rather large compared with the density of the ambient air, the Boussinesq approximation, which is typically used to analyze density currents, cannot be adopted in the present case. As opposed to previous models, the total buoyancy of a powder-snow avalanche is allowed to change freely via erosion from and deposition on to a static snow layer on a slope. In the model, the snow-particle entrainment rate from the slope is directly linked to the level of turbulence.A discontinuous, large-scale powder-snow avalanche occurred on 26 January 1986 near Maseguchi, Niigata Prefecture, Japan. The avalanche appears to have had a dense core at its base. The present model is employed to simulate that part of the avalanche above any dense core. The depth of the layer of fresh snow is considered to be an important parameter in the model. The larger the depth of fresh snow, the larger is the concentration of snow attained in the avalanche, and the faster its speed. It is seen that the model provides a reasonable description of the powder-snow avalanche generated near Maseguchi. In particular, the model prediction that a powder-snow avalanche strong enough to reach Maseguchi requires a depth of fresh snow of at least 2 m is in agreement with the observed depth just before the event.
ABSTRACT:In male rats challenged with the environmental estrogen bisphenol A, the compound is highly glucuronidated in the liver and is excreted largely into the bile. Given that in pregnancy the microsomal glucuronidation toward bisphenol A is attenuated, we hypothesized that elimination of bisphenol A from the liver may be reduced in pregnancy. This study was conducted to trace the elimination of bisphenol A in female rats, especially in pregnancy. In SpragueDawley rats, 1.5 mol of bisphenol A was perfused into the liver via the portal vein. In both the male and the nonpregnant female, the infused bisphenol A was glucuronidated, then the resultant glucuronide was excreted mainly into the bile. In pregnant rats, however, bilious excretion of bisphenol A glucuronide was 60% of that observed in nonpregnant rats, and venous excretion increased reciprocally. During 1-h perfusion, total excretion of the glucuronide from the liver of male, nonpregnant female, and pregnant rats was 889.5 ؎ 69.6, 1256.7 ؎ 54.8, and 1038.8 ؎ 33.3 nmoles, respectively. In Eisai hyperbilirubinemic rats (EHBR), perfusion of the liver with bisphenol A enabled us to determine that multidrug resistance-associated protein (MRP)2-mediating transport is the mechanism behind excretion of the glucuronide into the bile. The expression of MRP2 has been reported to be noticeably reduced in pregnancy. These results suggest that bisphenol A elimination by hepatic glucuronidation is slightly less in pregnancy than in nonpregnancy and that in pregnancy, more bisphenol A glucuronide is eliminated to the vein because of reduced MRP2 expression.
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