A new type of high‐pressure reactor based on liquid‐flow pressurisation using a HPLC pump has been developed. This high‐pressure reactor allows the easy and safe performance of reactions with gaseous alkanes under high‐pressures up to 10 MPa (100 atm), without the need for high‐pressure gas cylinders. The amount of substrate gas required for a single reaction is very small compared with reactions using a conventional autoclave, which, when using expensive substrate gasses, such as 13C‐labelled ethane, becomes critical. Employing this high‐pressure reactor in conjunction with cytochrome P450BM3 and the assistance of decoy molecules, the direct hydroxylation of gaseous alkanes was drastically improved. At 5 MPa the TOF of propane hydroxylation increased 10‐fold, reaching 2200 min−1. Hydroxylation of ethane was also substantially accelerated at 5 MPa, reaching a TOF of 28 min−1.
The front cover artwork for Issue 19/2019 is provided by Shoji and co‐workers at Nagoya University (Japan). The image shows the concept of their novel high‐pressure reactor which compresses gaseous alkanes by pumping liquid forcibly (the person wearing a T‐shirt) into the reaction vessel without an exit (the trunk), and pressurizes up to 10 MPa. See the Communication itself at https://doi.org/10.1002/cctc.201901323.
The Front Cover shows the concept of a novel high‐pressure reactor which compresses gaseous alkanes by pumping liquid forcibly (the person wearing a T‐shirt) into the reaction vessel without an exit (the trunk), and pressurizes up to 10 MPa. In their Communication, Yoshihito Watanabe, Osami Shoji, and co‐workers developed a novel high‐pressure reactor based on liquid‐flow pressurization using HPLC pump. Direct hydroxylation of gaseous alkanes by cytochrome P450BM3 with assistance of decoy molecules was drastically improved using this high‐pressure reactor. The TOF of propane‐hydroxylation reached 2200/min under 5 MPa of propane. Ethane hydroxylation was also greatly accelerated and the TOF reached 28/min. More information can be found in the Communication by Shinya Ariyasu, Yusaku Kodama, et al. on page 4709 in Issue 19, 2019 (DOI: 10.1002/cctc.201901323).
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