Here, we report the identification and functional characterization of the Streptomyces globisporus 1912 gene lndYR, which encodes a GntR-like regulator of the YtrA subfamily. Disruption of lndYR arrested sporulation and antibiotic production in S. globisporus. The results of in vivo and in vitro studies revealed that the ABC transporter genes lndW–lndW2 are targets of LndYR repressive action. In Streptomyces coelicolor M145, lndYR overexpression caused a significant increase in the amount of extracellular actinorhodin. We suggest that lndYR controls the transcription of transport system genes in response to an as-yet-unidentified signal. Features that distinguish lndYR-based regulation from other known regulators are discussed.
Aim. Screening of new natural bioactive molecules is one of the effective approach in combating multidrug-resistant pathogens and tumor cells. The aim of this work was to study the taxonomic characteristic, biological activity and secondary metabolites production of isolate Je 1-42 from the rhizosphere of Juniperus excelsa Bieb. Methods. Microbiological, molecular genetic, high performance liquid chromatography mass spectrometry and scanning electron microscopy methods were used in this work. Results. Based on a phylogenetic analysis of the 16S rRNA and gyrB gene sequences, microbiological and physiological characterization the isolate Je 1-42 has been affiliated to the genus Streptomyces. The isolate Je 1-42 showed a wide range of antibacterial activity. The antibiotics desertomycin A, kanchanamycin A, butylcycloheptylprodigiosine and spectinabilin were annotated in the Je 1-42 extract using dereplication analysis in the database DNP. Conclusions. Actinomycetes strain Streptomyces sp. Je 1-42 is an antagonist of gram-positive, gram-negative bacteria, fungi and produces antibiotics desertomycin A, canchanamycin A, butylcycloheptylprodigiozin, spectinabilin, which has a broad spectrum of antimicrobial activity. The production of these compounds obviously determines the ability of Je 1-42 to inhibit the growth of a wide range of bacteria and fungi. The strain of Streptomyces sp. Je 1-42 was deposited in the Microbial Culture Collection of Antibiotics Producers at Ivan Franko National University of Lviv (collection number Lv 1-042).
Keywords: Streptomyces, secondary metabolites, antibacterial activity, phylogenetic analysis.
The statistical evaluation of the H/V ratio of microseismic oscillations for determining the geotechnical properties of upper geological layer is proposed. Measurements have been carried out for different types of grounds and a good agreement of the results with its values of shear wave velocity is demonstrated. The proposed method is based on the usage of ultralow noise density 3-axis MEMS accelerometer, thus making the equipment very small and portable.
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