Two groups of viruses cause the citrus disease complex known as leprosis: the cytoplasmic type, of the genera Cilevirus and Higrevirus, and the nuclear type, of the genus Dichorhavirus. It has been shown that the cilevirus Citrus leprosis virus C (CiLV-C) is transmitted by Brevipalpus yothersi. Within the genus Dichorhavirus, CiLV-N is a recently described and distinct species present in Brazil and transmitted by B. phoenicis sensu stricto, whereas the species found in Mexico and Colombia are strains of Orchid fleck virus (OFV-citrus), suspected to be vectored by B. californicus. A study was conducted to determine whether B. californicus and B. yothersi can acquire and inoculate Mexican isolates of CiLV-C and OFV-citrus to sweet and acid citrus species (sweet orange, mandarin, grapefruit, sour orange, Persian lime and Mexican lime) in experiments set up in areas in Mexico with prevalence of the respective viruses. Brevipalpus californicus acquired OFV-citrus and transmitted it to all the citrus cultivars included in the experiment, while B. yothersi acquired CiLV-C and transmitted it only to sweet citrus cultivars (sweet orange, mandarin, and grapefruit). Both mite species were able to become established and reproduce in the experimental plants for nearly a year. This study represents the first experimental evidence of OFV-citrus transmission by B. californicus, as well as evidence that B. yothersi cannot transmit this virus.

Objectives: To generate information about a new host of the Polyphagotarsonemuslatus (Banks) mite, and to understand the damages caused by the cultivation ofApatlexco chili pepper (Capsicum annuum L.) in the northern region of the state ofGuerrero.Design/Methodology/Approach: The identification of the mite was carried out throughtaxonomic keys and the damages caused in vegetative shoots, mature leaves andflower buds were described.Results: The P. latus mite was identified as causing important damage to the crops ofAplaxtleco chili pepper grown in greenhouses in the state of Guerrero, Mexico, and thisis the first report of this mite in the Aplaxtleco chili pepper crop grown in greenhouses inthe state of Guerrero, Mexico.Findings/Conclusions: Economic income is obtained from the cultivation of Aplaxtlecochili peppers, a characteristic crop of the municipality of Aplaxtla, in the northern zone of Guerrero; therefore, knowing the identity of the P. latus mite in the Aplaxtleco chili cropwill help to suggest effective control methods to obtain higher yields.

The prickly pear cactus “tuna pelona” (Stenocereus zopilotensis), is an endemic cactus species from the state of Guerrero, Mexico, it develops in semi-arid areas and has great ecological importance as it houses and forms part of the diet of multiple species in its niche. There is a constant threat to natural populations of this species due to the overexploitation of seedling collections in the f ield by unregulated trade. In order to design an alternative for the conservation management of these species, the objective of the work was to evaluate treatments for the imbibition of “hairless tuna” seeds to promote germination and the successful establishment of seedlings with economic technologies for the farmers of the area. Fruits of "tuna pelona" (S. zopilotensis) were collected, near the community of Xalitla, municipality of Tepecoacuilco de Trujano, Guerrero; the seeds were stored for 70 and 273 days after collection (dac) at room temperature in order to maintain adequate conditions for the conservation of viability until reaching dormancy. In the stored seeds, the effect of soaking treatments was evaluated: 0, 6, 12, 24 and 12 hours in water plus another treatment that consisted of immersion in a plant-based hormonal stimulant. The germination percentage per treatment was evaluated, it was found that the control treatment (zero hours in water) of seeds stored at 70 dac only obtained 2.4% of germination. The treatment of soaking in water and in the hormonal stimulant both for 6 h presented the highest germination values with 48.6 and 44.4% respectively when using seeds of 273 dac at 14 days after the application of the treatments. These results will allow the massive production of seedlings of the species for reforestation and regional marketing purposes.

Objective: To evaluate three proportions of alcohol in traps with one and three windows and two different colors to capture Hypothenemus hampei Ferrari in Coffea arabica L. plants. Design/methodology/approach: The experiment followed a completely randomized design with a complete factorial arrangement and four replications. From March to July 2007, three mixtures of ethyl: methyl alcohol were evaluated in different proportions (1: 0, 0: 1 and 1: 1). In addition, green and transparent traps were used, designed with a single or three windows. The number of captured coffee berry borers, water loss, attractant evaporation and damaged fruits percentage was evaluated. The data were analyzed through an analysis of variance and a test of means differences (Tukey, p ? 0.05). Results: The mixture of ethyl: methyl alcohol 1: 1 was the best, with an average catch of 980 trapped - insects week-1. The single window of transparent color traps was the most efficient to avoid water loss, with an average loss of 1,129.79 mL and 905 mL respectively. Limitations on study/implications: The design and color of the traps did not influence the capture efficiency and the evaporation of the attractant during the evaluated months. Therefore, it should be further evaluated during other important phenological stages for the crop. Findings/conclusions: Transparent color traps with a window and 1: 1 ethyl: methyl alcohol were efficient for capturing H. hampei and lowering their population.

<p><strong>Background. </strong><em>Heterorhabditis</em> and <em>Steinernema</em> are two genera of entomopathogen nematodes used as effective biocontrollers in pest insect control. <strong>Target. </strong>To describe the morphometry, morphology and thus the molecular description of a new <em>Heterorhabditis</em> indica isolate, for the cultivation of sugarcane and its possible contamination against insect pests in Mexico. <strong>Methodology</strong>. This was obtained from soil samples through the technique of the insect bait <em>(Galleria mellonella</em>). Morphometric and morphological studies, in addition to molecular techniques (ITS1+5.8S+ITS2), were performed to correctly identify <em>H.</em><em> indica</em> CP13JA strain. This new strain of <em>H. indica</em> for the state of Veracruz was compared to <em>H. indica</em> originally described in India. <strong>Results.</strong> The presence of <em>H. indica</em> isolated from the sugar cane crop is reported in the state of Veracruz, Mexico. Likewise, the results obtained in this investigation identified as the characteristics of infectious juveniles, as well as amphitic males and females do not present differences in relation to the morphometry, morphometry and morphology of the original description; as well as in the molecular studies of the SU rDNA. <strong>Implications. </strong>Therefore, the presence of <em>H. indica</em> strain CP13JA is considered important within the sugarcane crop and for the state of Veracruz where it was isolated, as well as the potential that this isolation may have for the insect pest insects belonging that attack this crop<strong>. Conclusion.</strong> <em>H. indica</em> strain CP13JA does not present morphometric and molecular differences with the species recently described in India, in addition the presence <em>of H. indica</em> is considered new within the cultivation of sugar cane in Mexico</p>