TYE7, a bHLH (basic helix–loop–helix) transcription factor from Saccharomyces cerevisiae, is involved in the regulation of many genes, including glycolytic genes. Meanwhile, accumulating evidence indicates that TYE7 also functions as a cyclin and is linked to sulfur metabolism. Here, the structure of TYE7 (residues 165–291) complexed with its specific DNA was determined by X-ray crystallography. Structural analysis and comparison revealed that His185 and Glu189 are conserved in base recognition. However, Arg193 is also involved in base recognition in the structures that were compared. In the structure in this study, Arg193 in chain A has two conformations and makes a salt bridge with the phosphate backbone structure. In addition, a series of corresponding electrophoretic mobility shift assays were performed to better understand the DNA-binding mechanism of the bHLH domain of TYE7.
Background: Determining the factors associated with ENFI in neurocritical patients could be important for better identification of the patients at risk of feeding intolerance.Therefore, this study aimed to identify the risk factors for ENFI in neurocritical patients.Methods: Patients hospitalized in the neurological intensive care unit of the First Affiliated Hospital of University of Science and Technology of China between January 2021 and December 2021 were enrolled in this prospective case-control study.Result: Finally, 250 participants were enrolled. There were 183 patients in the early enteral nutrition feeding tolerance (ENFT) group and 67 patients in the ENFI group respectively. In the multivariable analysis, hypothermia treatment (OR = 2.331, 95% CI: 1.176-4.618, P = 0.015), severity of illness (OR = 2.176, 95% CI: 1.100-4.303, P = 0.025), the levels of sedation (OR = 2.478, 95% CI: 1.182-5.194, P = 0.016) and prealbumin (OR = 1.945, 95% CI: 1.073-3.525, P = 0.028) were independent risk factors of ENFI.Conclusion: Hypothermia treatment, severity of illness, levels of sedation and prealbumin were independent risk factors for ENFI.Chinese Clinical Trial Registry: ChiCTR2100044644
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