The tomato accession LA1996, which carries a dominant allele of anthocyanin fruit (Aft) locus, accumulates anthocyanins in the epidermis of fruits when exposed to sunlight. The involvement of blue, UV-A, UV-B and a combination of these wavelengths on anthocyanin accumulation and the molecular mechanism of their regulation was investigated in LA1996. The most effective treatment for inducing anthocyanin biosynthesis in Aft fruits was co-irradiation with blue and UV-B (blue + UV-B) light. Finding the correlated genes is an important approach towards understanding their molecular mechanisms.• In the present study, the nitrate reductase (NR) gene SlNIA was isolated using RNAseq profiling of Aft fruits given different light treatments. The functions of NR-mediated anthocyanin induction by blue + UV-B were confirmed using a series of chemical treatments, followed by assessment of NR activity and nitric oxide (NO) detection.• The expression of NR was highly induced by blue + UV-B, and this specificity was also confirmed with the enzyme activity of NR and the NO concentration. The NR inhibitors, which reduce NO generation, the expression levels of anthocyanin related genes and decreased anthocyanin accumulation in LA1996.• Our results suggest that NR plays a key role in blue + UV-B-mediated anthocyanin accumulation in LA1996 fruits.

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Anthocyanins are common secondary metabolites in plants that confer red, blue, and purple colorations in plants and are highly desired by consumers for their visual appearance and nutritional quality. In the last two decades, the anthocyanin biosynthetic pathway and transcriptional regulation of anthocyanin biosynthetic genes (ABGs) have been well characterized in many plants. From numerous studies on model plants and horticultural crops, many signaling regulators have been found to control anthocyanin accumulation via regulation of anthocyanin-promoting R2R3-MYB transcription factors (so-called R2R3-MYB activators). The regulatory mechanism of R2R3-MYB activators is mediated by multiple environmental factors (e.g., light, temperature) and internal signals (e.g., sugar, ethylene, and JA) in complicated interactions at multiple levels. Here, we summarize the transcriptional control of R2R3-MYB activators as a result of natural variations in the promoter of their encoding genes, upstream transcription factors and epigenetics, and posttranslational modifications of R2R3-MYB that determine color variations of horticultural plants. In addition, we focus on progress in elucidating the integrated regulatory network of anthocyanin biosynthesis mediated by R2R3-MYB activators in response to multiple signals. We also highlight a few gene cascade modules involved in the regulation of anthocyanin-related R2R3-MYB to provide insights into anthocyanin production in horticultural plants.

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