In this study, five novel Se-enriched antioxidant peptides
(FLSeML,
LSeMAAL, LASeMMVL, SeMLLAA, and LSeMAL) were purified and identified
from Se-enriched Moringa oleifera (M. oleifera) seed protein hydrolysate. The five peptides
showed excellent cellular antioxidant activity, with respective EC50 values of 0.291, 0.383, 0.662, 0.1, and 0.123 μg/mL.
The five peptides (0.025 mg/mL) increased the cell viability from
78.72 to 90.71, 89.16, 93.92, 83.68, and 98.29%, respectively, effectively
reducing reactive oxygen species accumulation and significantly increasing
superoxide dismutase and catalase activities in damaged cells. Molecular
docking results revealed that the five novel Se-enriched peptides
interacted with the key amino acid of Keap1, thus directly blocking
the interaction of Keap1-Nrf2 and activating the antioxidant stress
response to enhance the ability of scavenging free radicals in vitro.
In conclusion, Se-enriched M. oleifera seed peptides exhibited significant antioxidant activity and can
be expected to find widespread use as a highly active natural functional
food additive and ingredient.
A microgel-based biomimetic glutathione peroxidase with temperature responsive catalytic behavior is synthesized by integrating atom transfer radical polymerization (ATRP) technology into one-pot synthesis.
The development of selenium‐enriched food is one of the most effective ways to acheive selenium supplementation. However, the selenium content in selenium‐enriched food is unstable, and the selenium in the food needs to be incorporated into glutathione peroxidase (GPx) by the human body before it can exert its antioxidant activity. Herein, a selenium‐enriched cassava starch (Se‐ca‐starch80) with immediate antioxidant activity is synthesized by the successive esterification and selenation of cassava starch. The characterizations such as proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FT‐IR), scanning electron microscope (SEM), and X‐ray diffraction (XRD) indicate that the reactions mainly occur on the surface of starch and do not destroy the starch skeleton significantly. Such Se‐ca‐starch80 with selenium content of 9.44 µg g–1 reveals a remarkable antioxidant activity (the initial rate of the catalytic reaction, v0 > 10 µmol L–1 min–1) due to the cooperation of catalytic centers, substrate recognition sites, and hydrophobic microenvironments. Similar to the natural GPx, the Se‐ca‐starch80 is non‐cytotoxic and shows a saturation kinetic catalytic behavior. This work may open the door to design modified starch for functional foods and antioxidant drugs.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.