Abstract-Nowadays, with the widely use of cloud computing, the traditional forensics is relatively backward. The new forensic model and skills are needed in the new environment. On the basis of cloud computing, we research on the related technology, infrastructure and legal issues of live forensics in cloud environment and the data sources in cloud forensics are presented in the paper. Our mainly research is focus on providing the basic four processes according to the ISO/IEC 17043 and the latest methodologies of live forensics in cloud environment. At last, we proposed several measures to be further in-depth study in the future.
Canna yellow streak virus (CaYSV) is a potyvirus that causes severe damage to the ornamental plant canna in the United Kingdom and Brazil. Here we identified CaYSV in China by isolating total RNA from infected plant, amplifying the virus genome segments, cloning and sequencing the amplicons. After assembly, the full-length genome of the virus was obtained and uploaded to the NCBI database. Phylogenetic analysis results showed that the Guizhou isolate (OL546222) was most closley related to the KS isolate (MG545919.1). Virus detection is essential for virus disease control, but the subclinical infection of CaYSV on canna in its early development increases the difficulty of CaYSV diagnosis. The goal of this study was to develop an efficient method for detection of CaYSV. We designed the primers, optimized the reaction conditions, and finally established a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method. The product of RT-LAMP can be analyzed by both agarose gel electrophoresis and visible color change. The established one-step RT-LAMP assay showed high specificity and sensitivity in detecting CaYSV. This RT-LAMP method was also applied in analysis of 61 field samples collected from Guizhou and Jiangsu provinces. The results showed that the infection rates of CaYSV on canna samples from these two provinces were very high (63% and 96% respectively).
In the big data era, for us humans, forgetting have been the norm and remembering the exception. "Right to be forgotten and to erasure" which proposed by Data protection reform of EU got widely attention and controversy. This paper discussed the importance of the "right to be forgotten" for the protection of personal information, researched on the specific content of "Right to be forgotten and to erasure" in EUdraft, analyzed the legal status and defects of "right to erasure" in present stage of our country and proposed the legislation of "right to be forgotten", in order to improve the security of personal information online.
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