Enzyme mimics constitute a promising class of biocatalysts
with
potential applications in bioanalysis, biomedicine, etc. due to their
high catalytic activity and substrate specificity, but they often
suffer from low operational stability and difficulties in recyclable
utilization. Herein, we adopted an acid–base treatment to create
an efficient enzyme mimic material by taking advantage of biological
metal–organic frameworks (BioMOFs) constructed of the Cu2+ ion and a plant growth regulator. The prepared deprotonated
dinuclear BioMOF exhibits excellent stability in alkaline environments
and high peroxidase-mimicking activity. The enzyme mimic shows a quick
reaction capability of H2O2 scavenging in the
linear range of 0–0.2 μM mL–1 under
neutral conditions and a sustained scavenging ability under strong
alkaline conditions. Unlike general Cu-based peroxidase mimics, the
BioMOF directly hydrolyzes H2O2 into O2
2– instead of the •OH radical
and degrades to its synthetic precursor. The cyclic utilization, therefore,
can be achieved by the interconversion between the BioMOF and its
degraded product. Additionally, a hybrid nanomaterial (BioMOF-NPs)
was developed by immobilizing the BioMOF and chitosan nanoparticles.
The fabricated BioMOF-NPs display improved reliability and repeatability
of the H2O2-scavenging effect due to the large
specific surface area. Based on the BioMOF-NPs, selective glucose
determination is achieved in the linear range of 5–25 μM
with a detection limit of 1.93 μM. The method is successfully
utilized for the recyclable detection of glucose in apple fruits.
This study offers a new strategy for designing structure-interconvertible
BioMOFs for applications of biological analysis.
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