Previously, we reported that chromodomain Y-like (CDYL) acts as a crotonyl-coenzyme A hydratase and negatively regulates histone crotonylation (Kcr). However, the global CDYL-regulated crotonylome remains unclear. Here, we report a large-scale proteomics analysis for protein Kcr. We identify 14,311 Kcr sites across 3734 proteins in HeLa cells, providing by far the largest crotonylome dataset. We show that depletion of CDYL alters crotonylome landscape affecting diverse cellular pathways. Specifically, CDYL negatively regulated Kcr of RPA1, and mutation of the Kcr sites of RPA1 impaired its interaction with single-stranded DNA and/or with components of resection machinery, supporting a key role of RPA1 Kcr in homologous recombination DNA repair. Together, our study indicates that protein crotonylation has important implication in various pathophysiological processes. reveals CDYL-regulated RPA1 crotonylation in homologous recombination-mediated DNA repair.
Intelligent micromachines that respond to external light stimuli have a broad range of potential applications, such as microbots, biomedicine, and adaptive optics. However, artificial light-driven intelligent micromachines with a low actuation threshold, rapid responsiveness, and designable and precise 3D transformation capability remain unachievable to date. Here, a single-material and one-step 4D printing strategy are proposed to enable the nanomanufacturing of agile and low-threshold light-driven 3D micromachines with programmable shape-morphing characteristics. The as-developed carbon nanotube-doped composite hydrogel simultaneously enhanced the light absorption, thermal conductivity, and mechanical modulus of the crosslinked network, thus significantly increasing the light sensitivity and response speed of micromachines. Moreover, the structural design and assembly of asymmetric microscale mechanical metamaterial unit cells enable the highly efficient additive nanomanufacturing of 3D shape-morphable micromachines with large dynamic modulation and spatiotemporal controllability. Using this strategy, the world's smallest artificial beating heart with programmable light-stimulus responsiveness for the cardiac cycle is successfully printed. This 4D printing method paves the way for the construction of multifunctional intelligent micromachines for bionics, drug delivery, integrated microsystems, and other fields.
Quasi-1D
titanium trisulfide (TiS3) has strong in-plane
anisotropy with a direct band gap of about 1 eV, which has attracted
wide attention in the fields of microelectronics and optoelectronics.
However, the investigation of in situ synthesis,
synthetic mechanism, and nonlinear optical properties of TiS3 is rarely reported. In this work, we developed a transfer-free method
to grow TiS3 nanoribbons, successfully reducing the synthesis
time from a few days to less than 24 h without pretreatment. Raman
spectroscopy revealed TiS3 was not directly synthesized
by titanium (Ti) and sulfur (S) but by the further sulfurization reaction
of intermediate product TiS2. Moreover, the polarized four-wave
mixing (FWM) imaging of the as-grown TiS3 samples was conducted
by ultrafast nonlinear optical spectroscopy for the first time, identifying
the crystal axis orientation of TiS3 accurately and quickly.
Our work not only provides a rapid growth method for transfer-free
synthesis of TiS3 nanoribbons on SiO2/Si substrates
but also investigate the fast and non-destructive ultrafast nonlinear
optical characterization of quasi-1D TiS3, which lays a
foundation for understanding the synthetic mechanism and physicochemical
properties of transition metal trisulfides (TMTCs) and promoting the
functional device applications of TMTCs represented by TiS3.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.