Bio-electrokinetic remediation of oil-contaminated soil is a promising technology. In this study, three bio-electrokinetic remediation experiments were carried out to study the effects of external addition of oil-degrading bacteria by electrokinetics and different operational parameters on oil decontamination in saturated soil. Results showed that oil in soil migrated from the anode towards the cathode with forward electroosmotic flow and accumulated near the cathode. Oil was barely degraded without external addition of oil-degrading bacteria. Although electrolytes were refreshed every 12 hr, soil pH varied greatly at the electrodes under unidirectional operation. When electrode polarity was reversed every 2 hr, soil pH was efficiently controlled within the range of 6.35-9.75. The relative oil concentrations after the bidirectional experiment were in the range of 0.81-0.84 after 20 days of treatment. The relatively low oil degradation rate may be due to the facultative aerobic environment in the saturated soil matrix.
In this study, three bio-electrokinetic remediation experiments were carried out to study the external delivery of oil-degrading bacteria by electrokinetics and enzyme activities in saturated soil. Results showed that the oil-degrading bacteria could efficiently transport to the saturated oil-contaminated soil by electrophoresis and/or electroosmosis. Bacterial population increased from 6.02-7.75 log (CFU/g) in the control experiment to 8.53-12.83 log (CFU/g) after external bacterial delivery. Under unidirectional operation, the bacterial populations near the electrodes were lower than those in the middle of the soil as a result of unfavorable pH environments. In bidirectional experiment BEK-B, a relatively low bacterial population in the middle of the soil was observed after the experiment. The activities of catalase, polyphenol oxidase, and dehydrogenase have similar trends with bacterial population in the soil, indicating a good relation between the three enzymes and oil biodegradation of oil in soil.
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