We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive
and rapid Mycoplasma bovis detection. A total of 55 bacterial strains
were examined in this study, including 33 M. bovis strains, 14
non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains.
M. bovis was successfully detected by the LAMP assay within 60 min
without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab
samples were tested directly using our LAMP assays, the previously reported LAMP assay,
conventional PCR assay without pre-culture and comparing standard culture methods. The
improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively
(with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was
increased compared to existing methods.
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