Introduction.
Encephalitozoon pogonae is a newly described pathogen belonging to the phylum Microsporidia. In Austria and the USA, this species has been isolated from fatal and disseminated cases of captive-bred inland bearded dragons. Here, we report the case of fatal disseminated microsporidiosis caused by E. pogonae in two bearded dragons in Japan.
Case Presentation. The two lizards from different private households in Tokyo, Japan, had been brought to an animal hospital for examination. In both cases, the animal presented with a history of weight loss for several weeks. There were no improvements in clinical symptoms and the lizards deteriorated and finally died. Histopathological examination demonstrated necrotizing granulomatous inflammation attributed to disseminated microsporidian infection. Nucleotide sequencing of the nuclear ribosomal internal transcribed spacer region identified the microsporidian as E. pogonae with sequence identity of 100 %.
Conclusion. We report the first case, to our knowledge, of disseminated microsporidiosis caused by E. pogonae in inland bearded dragons in Japan. Although it is difficult to diagnose prenatally since the signs are nonspecific, the disease should be considered in the differential diagnosis of chronic infections that do not respond to antibiotics.
Differential phase contrast ͑DPC͒ scanning transmission electron microscopy was applied to investigate the magnetic structures of Fe granular films. The DPC images showed a cluster-like contrast of 200 nm in the lateral size, which could not be observed by Lorentz transmission electron microscopy in the Fresnel mode. The magnetization vector map reproduced from the DPC images indicated that a magnetization loop generally intersects several isolated particles of 20-40 nm in diameter. The films composed of densely dispersed magnetic particles showed larger magnetization loops, while more separately dispersed films tended to have smaller loops, or magnetization closures in one particle. The loop size is associated with the strength of magnetic interaction between magnetic particles.
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