DNA is an essential carrier of sequence-based genetic information for all life today. However, the chemical and physical properties of DNA may also affect the structure and dynamics of a vesicle-based model protocell in which it is encapsulated. To test these effects, we constructed a polyethylene glycol-grafted giant vesicle system capable of undergoing growth and division. The system incorporates a specific interaction between DNA and lipophilic catalysts as well as components of PCR. We found that vesicle division depends on the length of the encapsulated DNA, and the self-assembly of an internal supramolecular catalyst possibly leads to the direct causal relationship between DNA length and the capacity of the vesicle to self-reproduce. These results may help elucidate how nucleic acids could have functioned in the division of prebiotic protocells.
TheBiophysicalSociety of Japan General IncorporatedAssociation suggest that surface properties o uptake into a GUV, fPLGA-NPs are important for endoeytosis-like 3PTt40 Hirotaka Ariyama, Graduate Slrhooi qfStience and Tlechnology, Transportan permeation membranes using LUV suspension have been characteristics membranes remain unclear. 10 on membrane unitameltar vesic]e First, we investigated the dioleoylphosphatidylcholine(DOPC) containing the contrast, fiuorescence microscopy. DOPC-GUVsinduced the rapid monotonous leakage ofca]cein from of the most During the calcein leakage, the size ofmost GUVs decreased a litt]e (less than S %), but in some GUVs their radius decreased greatly and some small hlghcontrast particles appeared on the GUV membranes. The fraction of leaked GUVincreased with time, and aiso with an increase in TP 10 concentration, Seeond, the effect of cholesterol on the TP 1O-induced leakage ofca]cein was investigated. O.2 pM TP 10 did not induce ca]cein ]eakage from DOPCIchol (6I4)-GUVs. On the basis of these data, we discuss the mechan{sm of the interaction ofTP 1O with DOPC-GUVs and DOPC/chol-GUVs. FJyx nt-s ;t 1o pl Dopc utoE ± u utv-hopmmtatte tlliBt[ljZ6suM Effects of Transportan-10 on Membrane Permeability and Structure of Single Giant Unilame]lar Vesicles of DOPC membranes Masahito Yamuzaki (lntegrated Bio.seience Section, S]iizuoka Uhiv.) 10 {TP 10) is one of the cel] penetrating peptides,Se far the of TP 10 into cells and the inteTaction of TP 10 with ]ipid investigated. However, the detai] and mechanism of the interaction and its pemieation through In this report, we have investigated thc effeet of TP perrneability of lipid membranes using the sing]e giant (GUV)method. interaction of TP 1O with sing]e GUVs composed of and PEG2K-DOPE (mo]ar ratio, 98:2) fluorescent dye, calcein. in a physiological buffer using phase The interaction of O.8 pM TP lO with sing]e the inside GUVs, but in some GUVs step-wise leakages were observed. 3PT141 F-BARIISUUrtY-AOf=-IL,-YiYopU7JVStAMM Real-time observation ef liposome tubu]ation by F-BAR Yohko Takiguchii, Toshiki Itoh2, Kingo Takiguchii (iGrad. Sch. Sci., Uhiv, Nbgaya, 2Grad. Sch. Med., Uhiv. Kobe) The Fer-CIP4 homology-BAR (F-BAR) domain has been identit'ied as a biological membrane-deforming module. The F-BAR domain has been Teported to transform lipid bilayer membranes into tubules. However, the precess of tubutatien still remains unknewn, Here we monitored the entire tubulatien process indueed by the F-BAR domains or by the fu1Mengths of four different F-BAR dornain proteins, PSTPIPI, FBP17, CIP4 and Pacsin2, using direet rea]time irnaging, and show that each F-BAR domain or protein induces tubules through a unique k{netics ftem mode] membranes. FBPr7 and CIP4 develop many prejections simultaneous]y throughout the surfhce of indiividual liposomes, whereas PSTPIP1 and Pacsin2 deve]op on]y a few projections from a narrow restricted part of the surface of individuat ]iposornes. The resu]ts provide striking cvidcnce that a nucleation process is involvedint...
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