A β-lactams that act by inhibiting the bacterial cell wall biosynthesis are one of the most common classes of antibiotics applied to suppress the growth of latent bacterial infection associated with the plant tissue culture, as well as in the Agrobacterium-mediated transformation techniques. Plant sensitivity to antibiotics usually is species-, genotype-, or even tissue-specific and mainly depends on concentrations, growth conditions, and culture system. In the presented article, we estimated a comparative effect of four β-lactam antibiotics (Claforan®, timentin, amoxicillin, and Amoxiclav®) at different concentrations in an agar-solidified Murashige and Skoog (MS) culture medium supplemented with 5 mg L−1 6-benzylaminopurine (6-BA) and 0.1 mg L−1 indole-3-acetic acid (IAA) on in vitro callus induction and shoot organogenesis from hypocotyl and cotyledon explants of two tomato cultivars (Rekordsmen, Moryana). The role of clavulanic acid in combination with amoxicillin (Amoxiclav®) in the shoot organogenesis frequency and number of shoots per explant has been demonstrated. Additionally, the growth inhibition of Agrobacterium tumefaciens AGL0 strain according to agar disk-diffusion assay was studied. As a result, both stimulatory (timentin, amoxicillin, and Amoxiclav®) and inhibitory (Claforan®) effects of β-lactam antibiotics on in vitro morphogenetic responses of tomato were noted. It was found that clavulanic acid, which is part of the commercial antibiotic Amoxiclav®, significantly increased the shoot regeneration frequency from cotyledon and hypocotyl explants of Rekordsmen tomato cultivar. Possible reasons for the stimulating effect of clavulanic acid on the induction of shoot organogenesis are discussed. According to agar disk-diffusion assay, the maximum diameter of growth inhibition zones (43.9 mm) was identified using 200 mg L−1 timentin. The in vitro antibacterial activity of tested β-lactam antibiotics was arranged in the following order: timentin > Claforan® > amoxicillin ≥ Amoxiclav®. Thus, to suppress the growth of internal and latent bacterial infection of tomato plant tissue culture, as well as for transformation of Moryana and Rekordsmen cultivars by A. tumefaciens strain AGL0, we recommend adding of 100–200 mg L−1 timentin or 400–800 mg L−1 Amoxiclav® to the shoot induction medium.
The plants are an important part of the urban ecosystem. Plants help to reduce the level of pollution and the effect of adverse environmental factors on urban ecosystems. Among the most dangerous pollutants for plants are copper ions. Copper is one of the main pollutants of urban soil. Copper ions significantly reduce the quality of ornamental plants. One approach to solve this problem is to create plants resistant to adverse environmental factors using cell selection. This method is hardly ever used in urban greening. The aim of the work is to produce plants resistant to copper ions. It was developed technologies to obtain ornamental plants resistant to copper. These plants have substantially increased their resistance to copper. Plants Festuca rubra were resistant to high levels of copper contamination and can grow on the basis of the level of pollution along roads, in residential areas. Plants Brachycome iberidifolia proved to be resistant to the average level of pollution of copper and can grow in parks and squares.
Soil salinization is an important ecological problem in urban ecosystems and agro-ecosystems. Salinization has direct adverse effects on plants. Sodium chloride showed greater toxicity than bischofite to Festuca rubra. We used cell selection to increase the salt tolerance of the plants. We used sodium chloride in all stages of cultivation. We have obtained salt-tolerant Festuca rubra plants. Most Festuca rubra regenerants showed increased salt tolerance. Thus, biotechnological methods can be used to obtain lawn grasses that are resistant to salinity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.