Non-human primates (NHPs) are, due to their close phylogenetic relationship to humans, excellent animal models to study clinically relevant mutations. However, the toolbox for the genetic modification of NHPs is less developed than those for other species like mice. Therefore, it is necessary to further develop and refine genome editing approaches in NHPs. NHP pluripotent stem cells (PSCs) share key molecular signatures with the early embryo, which is an important target for genomic modification. Therefore, PSCs are a valuable test system for the validation of embryonic genome editing approaches. In the present study, we made use of the versatility of the piggyBac transposon system for different purposes in the context of NHP stem cell technology and genome editing. These include (1) Robust reprogramming of rhesus macaque fibroblasts to induced pluripotent stem cells (iPSCs); (2) Culture of the iPSCs under feeder-free conditions even after removal of the transgene resulting in transgene-free iPSCs; (3) Development of a CRISPR/Cas-based work-flow to edit the genome of rhesus macaque PSCs with high efficiency; (4) Establishment of a novel protocol for the derivation of gene-edited monoclonal NHP-iPSC lines. These findings facilitate efficient testing of genome editing approaches in NHP-PSC before their in vivo application.
The article deals with general analysis of the modern state of ensuring rights of Ukrainian citizens by law enforcement authorities in the sphere of firearms turnover and questions of establishment, perspectives of development The Firearms Act of Ukraine. There are deep social, economic and political transformations in Ukraine nowadays. It is necessary to declare, that progressive processes of construction of the legal democratic state, except the positive ones, have unavoidable adverse effects. So, the number of persons and refugees who have no means of subsistence and constant residences has increased. Accordingly number of crimes has increased, the activity of law enforcement structures have become lower, and they have no means to provide complete protection for private, municipal, state ownership, the rights of the person, as the freedom, honor and dignity of an individual from illegal acts.
The purpose of the article is to study computer crimes in the credit and financial sphere based on elements of forensic characteristics of crimes and analysis of the ways of their commission. The relevance of the study is due to the rapid increase in the number of computer crimes in the credit and financial sphere and the low level of their disclosure. The research was conducted using the method of system analysis and synthesis of information obtained from criminal proceedings, as well as reports from the Ministry of Internal Affairs of Ukraine and the National Police of Ukraine, from 2014 to 2018. The most actual motives and methods of committing computer crimes in the financial sphere have been analyzed and it has been established that during the period of Ukraine’s independence, the level of economic crimes has increased by almost 300%. The increase in the number of crimes contributes to the distrust of the injured party to the law enforcement agencies, savings of funds of financial institutions on cyber security, low level of information security of the financial sphere of Ukraine, lack of clear coordination between the relevant departments, which are responsible for the investigation of these crimes. The necessity of conducting separate investigative actions at the initial stage of the investigation has been justified in order to facilitate the rapid identification of the suspect, causing material damage and, in general, the investigation process.
Offenses, related to bribery and corruption, concern people and the state, since this form is a derivative part of crimes among officials. The purpose of the article is to study the issues of investigation and prevention of corruption crimes in the sphere of public administration. The urgency of this topic is that the diversity of forms of bribery, its penetration into various spheres of activity -economic, financial, entrepreneurial, education and show business -require new ways of preventing and counteracting these criminal manifestations, creating pragmatic recommendations aimed at improving their prevention and investigation
The article deals with a number of issues of investigation and prevention of corruption crimes in the field of public administration. The purpose of this paper is to analyze challenges in investigating and preventing corruption crimes in the field of public administration. The relevance of this study lies in the fact that the variety of forms of bribery, its penetration into various spheres of activity: economic, financial, entrepreneurial, educational, requires new ways to prevent and counteract these criminal manifestations, the creation of pragmatic recommendations aimed at improving their prevention and investigation. The study was carried out based on the method of system analysis and generalization of information obtained in the course of the study, questionnaires of different categories of law enforcement officers. They conduct pre-trial investigation of these crimes, as well as reports of the Expert Service of the Ministry of Internal Affairs of Ukraine, the National Police of Ukraine, the National Anti-Corruption Bureau of Ukraine, and Forensic Science Institutes of the Ministry of Justice of Ukraine for 2016-2019, the legal framework on liability for corruption offenses. The most relevant motives and methods of committing corruption crimes have been analyzed and it has been established that bribery and corruption rank first among economic crimes, and the high level of corruption of state bodies in various spheres of public life contributes to the increase in the number of such crimes. The study found that civil servants through abuse of office, as well as obtaining undue benefits predominantly commit corruption crimes. The ways of improving the forms of combating corruption in public authorities are proposed, which will reduce the level of corruption in the public administration system, in particular, the implementation of measures aimed at enhancing information exchange between non-governmental organizations, the media, the public and local authorities, and public authorities.
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