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Human fibrinogen-related protein-1/liver fibrinogen-related protein-1 (HFREP-1/LFIRE-1), a liverspecific protein, is a member of fibrinogen superfamily that exerts various biological activities. However, the function of HFREP-1/LFIRE-1 in liver remains unknown. Here we isolated its mouse ortholog gene-mouse fibrinogen-related protein-1 (mfrep-1), which encoded 314 amino acids, exhibiting 80.4% similarity to HFREP-1/LFIRE-1. Northern blot analysis revealed that 1.2-kb mfrep-1 mRNA was detected selectively in mouse liver. To explore the function of MFREP-1, we examined the levels of mfrep-1 mRNA during regeneration after 70% partial hepatectomy (PHx) in mice. mfrep-1 mRNA increased in the regenerating liver and reached the first shoulder peak at 2-4 h after PHx. Cycloheximide pretreatment could suppress the induction of mfrep-1, indicating the up-regulation of this gene need de novo protein synthesis. Its mRNA continued to elevate at 6 h thereafter and reached the second peak at 24 h. The enhanced expression of mfrep-1 maintained high until 72 h and then declined slowly to the basal level. Immunohistochemistry assessment confirmed the up-regulated expression of MFREP-1 protein in parenchymal cells during liver regeneration. These data suggested that MFREP-1 might play an important role in liver regeneration and be involved in the regulation of cell growth.Key words: mfrep-1, in silico cloning, liver regeneration, liver-specific expression.

Ti matrix composites reinforced with 0.6 wt.% reduced graphene oxide (rGO)

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Aluminum 6061 matrix composite reinforced by 35 wt% B 4 C particle was fabricated by power metallurgy method. Then, the as-deformed composite was tested by quasi-static (0.001 s-1) and dynamic (760-1150 s-1) compression experiments. The Johnson-Cook plasticity model was employed to model the flow behavior. The damage mechanism of composite was analyzed through the microstructure observations. The results showed that the B 4 C particles exhibited uniform distribution and no deleterious reaction product Al 4 C 3 was found in the composite. Al6061/B 4 C composite showed high yield strength, moderate strain rate sensitivity and strain hardening under the dynamic loading, and a constitutive model under dynamic compression was established based on Johnson-Cook model, and accorded well with experimental results. The microstructure damage was dominated by particle fracture and interface debonding, and the dislocation was observed in the composite at a higher strain rate.