We develop a simplified light source at 461 nm for laser cooling of Sr without frequency-doubling crystals but with blue laser diodes. An anti-reflection coated blue laser diode in an external cavity (Littrow) configuration provides an output power of 40 mW at 461 nm. Another blue laser diode is used to amplify the laser power up to 110 mW by injection locking. For frequency stabilization, we demonstrate modulation-free polarization spectroscopy of Sr in a hollow cathode lamp. The simplification of the laser system achieved in this work is of great importance for the construction of transportable optical lattice clocks.
Caveolae are invagination structures in plasma membrane, size of about 100 nm. The main function of caveolae is still under debate. Recently it is proposed that the caveolae become flattened as buffers of plasma membrane at hypo-osmotic shock. We performed theoretical analysis and computer simulation to test this hypothesis. In the theoretical analysis, we calculated force acted on the caveolae and found that, the direction of force depends on the shape of caveolae: the expanding force and constriction force are both expected. The constriction force may give the opposite result; constricted caveolae. Then we performed coarse-grained membrane (polygon membrane) simulation and confirmed the existence of constricted caveolae, which is a novel membrane structures. 2P194細胞性粘菌の F-アクチン波の基質依存性 F-actin waves that propagate at the membrane cortex are known in a variety of moving cells. In Dictyostelium, waves of PIP3/F-actin are enriched in PI3Kinase and Arp2/3 complex thus appear related to leading edge structure of migratory cells. However, the exact conditions supporting waves and their link to cell migration remains elusive. Here, by combining confocal and TIRF microscopy, we demonstrate that wave nucleation in Dictyostelium is substrate-dependent. The PIP3/F-actin waves are suppressed on a poly-lysine coated coverglass or PDMS. The suppression is negated by treating PDMS with air plasma, which renders the substrate negatively charged. We will discuss the relation between strength of cellsubstrate attachment, its spatio-temporal change and actin-waves. 2P195 心筋細胞における温度依存的拍動周期の特性Characterization of temperature-dependent beating rate of cardiomyocytes Tomoyuki Kaneko (LaRC, Dept. Frontier Biosci., Hosei Univ.) Cardiomyocytes are known to change the beating rate depending on temperature. To characterize the beating rate of cardiomyocytes with changing temperature, we measured the inter-spike interval (ISI) of cardiomyocytes derived from chick embryo (E13) by multi-electrode array (MEA) system. The beating rate was not increased immediately after starting the temperature rises and was increased sigmoidal as temperature rises from 15°C to 32°C after lag time of several minutes. The stationary phase of the beating rate was constant at repeated procedure of three times. These results suggested that cardiomyocytes could possess an inherent beating rate corresponding to surrounding environments. 2P196 多電極電位計測による心筋細胞に対するエタノールの影響 Effect of ethanol on cardiomyocytes measured by multielectrode array systemChiho Nihei, Tomoyuki Kaneko (LaRC, Dept. Frontier Biosci., Hosei Univ.)Ethanol is contained in liquor and had various influence to human body. To examine the effect of ethanol to heart, we applied ethanol to cardiomyocytes derived from chicken embryo (E13). We measured inter spike interval (ISI) and field potential duration (FPD) of the cells by multielectrode array system. A low concentration of ethanol (0.1%) had no apparent effect on both ISI and FPD. As shortening of ISI and FPD occurred at the concentration of more than ...
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