Minor constituents were isolated from a mixture of commercial oleic acid manufactured from beef tallow by 10 different companies. Silicic acid was used as an adsorbent to isolate the minor constituents. They were first separated into acidic and nonacidic fractions. Each fraction was then separated into numerous subfractions by stepwise gradient elution liquid chromatography, using silicic acid as the adsorbent. The subfractions which had an adverse effect on the color stability of oleic acid during heating were characterized with functional group analysis, elemental analysis and IR spectrophotometry. The minor constituents amounted to ca. 1.18% of the commercial oleic acid. They were complex mixtures with multiple functional groups. Some of the subfractions contained 2\s‐3 times as much oxygen in the molecule as oleic acid. They had molecular weights ranging from 308 to 830 which are from monomers to trimers of oxidized oleic acid. These minor components contained carbonyl, ester and hydroxyl groups and double bonds. Some of the nonacidic minor constituents may contain amide groups. Most of the acidic subfractions were dark red, viscous liquids and the nonacidic subfractions were dark green or greenish‐brown, semisolid substances. A relationship was established that the greater the polarity of the minor constituents, the greater is its adverse effect on the color stability of oleic acid during heating.
, 293 (1982) 7) •gStandard Methods for the Analysis of Oils, Fats and Derivatives•h, Japan Oil Chemists' Society 2.4.5.1-71; 2.4.1-83; 2.4.20-71; 2.4.21-73 8) •gStandard Methods for the Analysis of Oils, Fats and Derivatives•h, Japan Oil Chemists' Society 2.3.
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