In these reports the separation were obtained by using specimens or deionized sample of NeuAc-containing compounds and the resolution was attained with acidic diluted phosphate or acetate buffer.We chose serotonin-immobilized silica as solid phase extraction (SPE) particles for NeuAc-containing glycoconjugates, 2010 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. E-mail: suzuki@phar.kindai.ac.jp specific Extraction of sialic-acid-containing Glycans and Glycopeptides using serotonin-bonded silica Masahiro Yodoshi, tomoko ikuta, Yukie Mouri, and shigeo suzuki † Department of Pharmaceutical Sciences, Kinki University, Japan Serotonin-bonded silica was developed for specific extraction of sialic-acid-containing glycans and glycopeptides. Serotonin, coupled by reductive amination with aldehyde silica particles via its ethylamino group, has strong affinity to sialic acid in glycan chain termini. Sialylated glycans trapped on serotonin silica particles are released by washing with ammonium acetate solution, providing highly efficient specific trap and release glycoconjugates. With >100 μmol/g adsorption capacity, the particles are applicable to purify labeled glycans after derivatization with 2-aminopyridine to remove excess reagents. Serotonin silica efficiently enriches sialic-acid-containing glycopeptides from tryptic digests for LC/MS analysis of glycans' heterogeneity in glycoproteins.
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