IntroductionIn this study aimed to discuss the importance of the combination of cultural heritage management and green development for urban development by analyzing the upgrading and renovation of the Grand Canal (Hangzhou section) as a successful case. In recent years, green development has risen to prominence as a paradigm shift. Additionally, culture, as an engine to drive urban development, has received more attention.MethodsThis research used a hybrid approach to examine the importance of combining green development with cultural heritage management. The qualitative method was an interview analysis of 13 residents living in the Hangzhou section of the Grand Canal. Based on the analysis of multiple water quality variables in Hangzhou from 1998 and 2014 to 2021, the empirical results proved that it is feasible to integrate green development (environment and economy) into the cultural heritage management of the case study area.Results and discussionThe results further prove that only through an understanding of the relationship between cultural heritage and green development can a virtuous cycle of development be created, thereby promoting the continuous development of a unique and historically significant urban area. The results of this study suggest that, in the development of mega-cities, although the preservation and inheritance of historical and cultural heritage conflicts with the green development of modern cities, a successful example has been explored in Hangzhou, including grassroots governance efforts like Gongshu District. There, the two factors can be mutually compatible and promote each other, enhancing the well-being and happiness of local residents.
microRNAs (miRNAs) are small endogenous RNAs composed of 20-22 nucleotides that do not encode proteins, which regulate the expression of downstream genes by targeting the 3’ untranslated region of mRNA. Plentiful research has demonstrated that miRNAs participate in the initiation and development of diverse diseases and malignant tumors. miR-1258 exerts great influence on tumors, including tumor growth, distant metastasis, migration, invasion, chemosensitivity, cell glycolysis, apoptosis, and stemness. Interestingly, miR-1258 is a miRNA with explicit functions and has been investigated to act as a tumor suppressor in studies on various types of tumors. With accumulating research on miR-1258, it has been found to be used as a biomarker in the early diagnosis and prognosis prediction of tumor patients. In this review, we outline the development of miR-1258 research, describe its regulatory network, and discuss its roles in cancer. Additionally, we generalize the potential clinical applications of miR-1258. This review offers emerging perspectives and orientations for further comprehending the function of miR-1258 as a diagnostic and prognostic biomarker and potent therapeutic target in cancer.
Background: Molecular-genetic imaging greatly advances clinical diagnosis and prognosis monitoring. However, specific visualization of cytoplasmic molecules remains an important but elusive goal. Estrogen receptor (ER) and progesterone receptor (PR) as the intracellular proteins and crucial markers of breast cancer determine the therapeutic regimes and prognosis of breast cancer patients. Methods: Here, we elegantly demonstrate that the ER/PR responsive elements can be genetically engineered to drive imaging reporters selectively and to enable the detection of ER/PR positive tumors using a far-red light photoactivatable near-infrared fluorescent protein. The specific molecular imaging probe were transfected into the cells and solid tumors using cationic polymers for lighting ER/PR breast cancers via a near-infrared fluorescence signal. Luciferase reporter system, ER/PR antagonist treatment, immunoblotting, and immunohistochemistry were performed to confirm the molecular mechanisms underlying the steroid receptor probe. Results: Notably, the double responsive elements of ER/PR exhibit the most response sensitivity to the steroid receptors in the breast cancers. By leveraging a cationic polymer vector, the construct consisting of optimal responsive elements and fluorescence protein can locally image the ER/PR positive breast cancers in murine models under a near-infrared laser, achieving non-invasive diagnosis of the steroid receptor positive cancers. No death or serious anaphylactic activity was observed in the animals during the study. Conclusion: As a result of its ER/PR specificity and potential for the clinical translation, the reporter system consisting of the steroid receptor response elements and near-infrared proteins might provide a novel practical system for identifying biomarkers, and advancing cancer diagnosis as well as therapy.
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