Acute autonomic and sensory neuropathy is a rare disorder that has been only anecdotally reported. We characterized the clinical, electrophysiological, pathological and prognostic features of 21 patients with acute autonomic and sensory neuropathy. An antecedent event, mostly an upper respiratory tract or gastrointestinal tract infection, was reported in two-thirds of patients. Profound autonomic failure with various degrees of sensory impairment characterized the neuropathic features in all patients. The initial symptoms were those related to autonomic disturbance or superficial sensory impairment in all patients, while deep sensory impairment accompanied by sensory ataxia subsequently appeared in 12 patients. The severity of sensory ataxia tended to become worse as the duration from the onset to the peak phase of neuropathy became longer (P<0.001). The distribution of sensory manifestations included the proximal regions of the limbs, face, scalp and trunk in most patients. It tended to be asymmetrical and segmental, rather than presenting as a symmetric polyneuropathy. Pain of the involved region was a common and serious symptom. In addition to autonomic and sensory symptoms, coughing episodes, psychiatric symptoms, sleep apnoea and aspiration, pneumonia made it difficult to manage the clinical condition. Nerve conduction studies revealed the reduction of sensory nerve action potentials in patients with sensory ataxia, while it was relatively preserved in patients without sensory ataxia. Magnetic resonance imaging of the spinal cord revealed a high-intensity area in the posterior column on T(2)*-weighted gradient echo image in patients with sensory ataxia but not in those without it. Sural nerve biopsy revealed small-fibre predominant axonal loss without evidence of nerve regeneration. In an autopsy case with impairment of both superficial and deep sensations, we observed severe neuronal cell loss in the thoracic sympathetic and dorsal root ganglia, and Auerbach's plexus with well preserved anterior hone cells. Myelinated fibres in the anterior spinal root were preserved, while those in the posterior spinal root and the posterior column of the spinal cord were depleted. Although recovery of sensory impairment was poor, autonomic dysfunction was ameliorated to some degree within several months in most patients. In conclusion, an immune-mediated mechanism may be associated with acute autonomic and sensory neuropathy. Small neuronal cells in the autonomic and sensory ganglia may be affected in the initial phase, and subsequently, large neuronal cells in the sensory ganglia are damaged.

There are high prevalence rates for PD and APS and suboptimal recognition of APS. This is the first epidemiological prevalence study of MSA from Japan.

With the increasing development of electric vehicles and portable devices, there is a strong requirement for high-energy batteries. To improve battery energy, multielectron transfer electrode reactions can be applied. Previously, batteries based on fluoride-ion shuttle (F– ion shuttle batteries, FiBs) have been reported, utilizing electrodes with multielectron transfer reactions. Although these FiBs exhibit high theoretical energy densities, reported capacities are significantly less than theoretical values. Moreover, charge–discharge mechanisms are not clarified. In this study, the feasibility of FiBs as extremely high-energy batteries has been demonstrated using a model cell with a Cu cathode and a LaF3 anode. By conducting experiments under an atmosphere without impurities, the Cu/LaF3 battery has been successfully operated with almost theoretical capacity. The Cu/LaF3 battery has been exhibited a superior cycle life at 80 °C, with feasibility for room-temperature operation.

Fluoride-shuttle batteries (FSBs), which are based on fluoride-ion transfer, have attracted attention because of their high theoretical energy densities. The fluorination and defluorination reactions at the electrodes are the possible rate-determining steps in FSBs, and understanding the mechanism is important to achieve smooth charge/discharge. In this study, we discuss the thermodynamically favored pathways for the fluorination and defluorination reactions and compare the reactions through the solid-solution and two-phase-coexistent states by density functional theory (DFT) calculations. The free energies of the solid-solution and two-phase states approximate the energies calculated by DFT, and their accuracy was validated by comparison with experimental formation enthalpies and free energies. The relative formation enthalpies of typical, transition, and relativistic metal (Tl, Pb, and Bi) fluorides are well reproduced by DFT calculations within 0.1, 0.2, and 0.4 eV, respectively. We also show that the reaction pathway can be determined by comparing the formation enthalpies of the metal fluoride H, a fluorine vacancy H V, and an interstitial fluorine defect H I from the simple selection rule. The enthalpy relation of H I > H > −H V observed in all the calculations strongly suggests that fluorination and defluorination in FSB electrodes occur by a two-phase reaction. This fluorination and defluorination mechanism will be useful to clarify the rate-determining step in FSBs.

Among four closely related members of the FGF receptor family, FGFR 1, 2, and 3 have alternative splicing forms encoded by different exons for the C-terminal half of the third Ig-like domain, but FGFR 4 has no such alternative exon. Furthermore, FGFR 1, 2, and 3 have another splice variant of nontransmembrane type; however, such a variant has not been reported for FGFR 4. While searching for a novel receptor-type tyrosine kinase by RT-PCR, we identified a non-transmembrane-type receptor of FGFR 4 in human intestinal epithelial cell lines (Intestine 407 and Caco-2). Sequence analysis of this receptor revealed that exon 9 coding the single transmembrane domain was displaced by intron 9. Consequently, this variant form was 120 bp shorter than the normal form and had no transmembrane portion. Moreover, the signal sequence in exon 2 was maintained, suggesting that this splice variant is a soluble receptor. This soluble receptor was detected in human gastrointestinal epithelial cells and pancreas, and also in gastric, colon, and pancreatic cancer cell lines. Single cell RT-PCR showed that this soluble receptor was expressed simultaneously with the transmembrane-type receptor in the same cell. Western blot analysis revealed that this receptor was secreted from the transfected COS7 cells. Thus, a soluble-form splice variant of FGFR 4 was identified in human gastrointestinal epithelial cells and cancer cells. This is the first report of alternative splicing of FGFR 4.