To improve the energy prediction performance of a building energy model, the occupancy status information is very important. This is more important in real buildings, rather than under construction buildings, because actual building occupancy can significantly influence its energy consumption. In this study, a machine learning based framework for a consecutive occupancy estimation is proposed by utilizing internet of things data, such as indoor temperature and luminance, CO2 density, electricity consumption of lighting, HVAC (heating, ventilation, and air conditioning), electric appliances, etc. Three machine learning based occupancy estimation algorithms (decision tree, support vector machine, artificial neural networks) are selected and evaluated in terms of the performance of estimating the occupancy status for each season. The selection process of the input variables that have crucial impact on the algorithms’ performance are described in detail. Finally, an occupancy estimation framework that can repeat model training and estimation consecutively in a situation when time-series data are continuously provided over the entire measurement period is suggested. In addition, the performance of the framework is evaluated to identify how it improves the energy prediction performance of the building energy model compared to conventional energy modeling practices. The suggested framework is distinguished from similar previous studies in two ways: 1) The proposed framework reveals that input variables for the occupancy estimation model can be occasionally changed by an occupant response to certain times and seasons, and 2) the framework incorporates time-series indirect occupancy sensing data and classification algorithms to consecutively provide occupancy information for the energy modeling effort.
We proposed a method of label-free segmentation of cell nuclei by exploiting a deep learning (DL) framework. Over the years, fluorescent proteins and staining agents have been widely used to identify cell nuclei. However, the use of exogenous agents inevitably prevents from long-term imaging of live cells and rapid analysis and even interferes with intrinsic physiological conditions. Without any agents, the proposed method was applied to label-free optical diffraction tomography (ODT) of human breast cancer cells. A novel architecture with optimized training strategies was validated through cross-modality and cross-laboratory experiments. The nucleus volumes from the DL-based label-free ODT segmentation accurately agreed with those from fluorescent-based. Furthermore, the 4D cell nucleus segmentation was successfully performed for the time-lapse ODT images. The proposed method would bring out broad and immediate biomedical applications with our framework publicly available.INDEX TERMS Cell nucleus segmentation, deep learning, label-free segmentation, optical diffraction tomography, refractive index tomogram.
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