The transcription factor ELONGATED HYPOCOTYL5 (HY5) plays critical roles in plant photomorphogenesis. Previous studies on HY5 have mainly focused on the seedling stage in Arabidopsis (Arabidopsis thaliana), and its functions in other plant species have not been well characterized, particularly at adult stages of development. In this report, we investigated the functions of tomato (Solanum lycopersicum) HY5 (SlHY5) from seedlings to adult plants with a focus on fruits. Genome-edited slhy5 mutants exhibited typical compromised photomorphogenesis in response to various light conditions. The slhy5 mutants showed reduced primary root length and secondary root number, which is associated with altered auxin signaling. SlHY5 promoted chlorophyll biosynthesis from seedling to adult stages. Notably, the promotive role of SlHY5 on chlorophyll accumulation was more pronounced on the illuminated side of green fruits than on their shaded side. Consistent with this light-dependent effect, we determined that SlHY5 protein is stabilized by light. Transcriptome and metabolome analyses in fruits revealed that SlHY5 has major functions in the regulation of metabolism, including the biosynthesis of phenylpropanoids and steroidal glycoalkaloids. These data demonstrate that SlHY5 performs both shared and distinct functions in relation to its Arabidopsis counterpart. The manipulation of SlHY5 represents a powerful tool to influence the two vital agricultural traits of seedling fitness and fruit quality in tomato.
Objective There is no effective standard method to evaluate whether the nerve root tension is restored during lumbar decompression surgery, which is an important indicator for the recovery of nerve function. This study aimed to investigate the feasibility of intraoperative nerve root tension measurement and to confirm the correlation between nerve root tension and intervertebral space height. Methods A total of 54 consecutive patients (mean age, 54.3 years; range, 25–68 years) received posterior lumbar interbody fusion (PLIF) for lumbar disc herniation (LDH) with lumbar spinal stenosis and instability. The 110%, 120%, 130%, 140% height values of each lesion were calculated based on preoperative measurements of the intervertebral space height. The heights were intraoperatively expanded after the intervertebral disc was removed using the interbody fusion cage model. The tension value of nerve root was measured by pulling the nerve root for 5 mm with a self‐made measuring device. The nerve root tension value was measured before decompression, after discectomy at 100%, 110%, 120%, 130%, and 140% of the height of each intervertebral space, and after placement of the cage during intraoperative nerve root tension monitoring. Results The nerve root tension values at 100%, 110%, 120%, and 130% heights were significantly lower than those before decompression, and there was no statistical significance among the four groups. The nerve root tension value was significantly higher at 140% height and was statistically significant compared with that of 130% height. The nerve root tension value after cage placement was significantly lower than that before decompression (1.32 ± 0.22 N vs. 0.61 ± 0.17 N, p < 0.01), and the postoperative VAS score was also significantly improved (7.0 ± 2.24 vs. 0.8 ± 0.84, p < 0.01). The nerve root tension was positively correlated with the VAS score (F = 85.19, p < 0.01; F = 78.65, p < 0.01). Conclusion This study demonstrates that nerve root tonometry can perform instant noninvasive intraoperative nerve root tension measurement. There is a correlation between nerve root tension value and VAS score. We found that when the height of the intervertebral space was increased to 140% of the original height, the nerve root tension increased the risk of injury significantly.
Although green light (GL, 500–600 nm) occupies half the visible light spectrum and regulates a series of plant developmental processes, the mechanism by which GL regulates seedling morphogenesis is enigmatic. Here, we reported that pure GL (500–600 nm, λmax, 527 nm) promoted the cotyledon development of Arabidopsis seedlings through phytochrome B (phyB). Genetic analysis indicated that phyB was involved in cotyledon development under continuous GL. Compared with red light (RL), GL induced phyB translocation from the cytoplasm to the nucleus and speckle-like photobody formation with a slower rate, which was reversed by far red light. Further transcriptomic data demonstrated that phyB participated in GL-responsive transcriptional networks in concert with four PHYTOCHROME-INTERACTING FACTORS (PIFs, PIF1, PIF3, PIF4, and PIF5). As expected, protein levels of the PIFs were decreased by GL. Similar to RL, GL induced the rapid degradation of PIF3 in a phy-dependent manner. Our results suggest that pure GL (527 nm) leads to the migration of phyB into the nucleus to trigger photomorphogenesis by possibly promoting the degradation of PIFs.
Background This study explored the therapeutic effects of transplantation of neural stem cells (NSCs) encapsulated in hydrogels in a cauda equina lesion model.Methods NSCs were isolated from neonatal dorsal root ganglion (DRG) and cultured in three-dimensional porous hydrogel scaffolds. Immunohistochemistry, transmission electron microscopy, Luxol fast blue staining, TUNEL assay were performed to detect the differentiation capability, ultrastructural and pathological changes, and apoptosis of NSCs. Furthermore, the functional recovery of sensorimotor reflexes was determined using the tail-flick test.Results NSCs derived from DRG were able to proliferate to form neurospheres and mainly differentiate into oligodendrocytes in the three-dimensional hydrogel culture system. After transplantation of NSCs encapsulated in hydrogels, NSCs differentiated into oligodendrocytes, neurons or astrocytes in vivo . Moreover, NSCs engrafted on the hydrogels decreased apoptosis and alleviated the ultrastructural and pathological changes of injured cauda equina. Behavioral analysis showed that transplanted hydrogel-encapsulated NSCs decreased the tail-flick latency and showed a neuroprotective role on injured cauda equina.Conclusions Our results indicate transplantation of hydrogel-encapsulated NSCs promotes stem cell differentiation into oligodendrocytes, neurons or astrocytes and contributes to the functional recovery of injured cauda equina, suggesting that NSCs encapsulated in hydrogels may be applied for the treatment of cauda equina injury.
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