Objective: To examine the effect of filler contentTo examine the effect of filler content on structural coloration of experimental flowable resin composites. Overview: Experimental composites containing spherical fillers and different filler contents (30, 40, 50, 60 and 70 wt%), a structural colored resin composite (Omnichroma), and a nanohybrid flowable resin composite (Gracefil LoFlo) were used. The optical characteristics of the specimens were measured on standard black and white backgrounds using a spectrophotometer under CIE D65 illuminant and the L*a*b* values were determined. The color matching abilities to artificial teeth were evaluated using the ΔE*ab and ΔE00 color difference formulas. One-way analysis of variance and Tukey's post hoc test were used. The spectral reflectance of experimental composites showed different trends in different backgrounds, plate thickness, and filler contents. In particular, higher spectral reflectance was observed on the white background at wavelengths ranging from 450 to 700 nm in both 1.5 and 3.0 mm plates when compared to the black background. The percent reflectivity tended to decrease with a decrease of the filler contents. The color matching abilities of the experimental composites improved with the increase in the filler contents. Conclusions: Structural coloration of the flowable resin composites were affected by the filler contents. Clinical significance: Clinical Significance: Higher filler contents might be needed to achieve the optimal color properties of structural colored flowable resin composites, thereby improving the restoration esthetics.