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In August 2020, a new fungal disease was observed on strawberry in Shandong Province, which showed that small spots of grayish‐black, near round without lesions and haloes on the adaxial of strawberry leaves. The morphological features of colonies and conida on PDA medium were consistent with Colletotrichum gloeosporioides species complex. The rDNA internal transcribed spacer, calmodulin, chitin synthase, and actin from the isolates were amplified and sequenced. BLAST analysis of these four genes showed 99.24‐100.00% identity with the corresponding sequences of C. siamense in GenBank. The result of phylogenetic analysis also indicated that the pathogen was identified as C. siamense. Similar symptoms were observed on the back of strawberry leaf after spraying conidial suspensions for 3 days, and C. siamense was reisolated which confirm the Koch's rule. This is the first report of new disease of black leaf spot on strawberry caused by C. siamense.

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