Pre-pregnancy overweight or obesity is associated with increased risk of adverse pregnancy outcomes. Nutrition counseling is recommended before pregnancy in women who have overweight or obesity.
Dendrobium
is known for its pharmacological actions including anti-cancer effect, anti-fatigue effect, gastric ulcer protective effect, and so on. At present, only studies on endophytic fungi of
Dendrobium
affecting the metabolites of host plants have been reported, very little research has been done on endophytic bacteria. In this study, we have demonstrated the great diversity of endophytic bacteria in 6
Dendrobium
samples from different origins and cultivars. According to the results of the culture-independent method, the endophytic bacterial community in
Dendrobium
stems showed obvious different in the 6 samples and was influenced by origin and cultivar. Some bacteria including
Ralstonia
,
Comamonas
and
Lelliottia
were first detected in
Dendrobium
in this study. Based on the culture-dependent method, a total of 165 cultivable endophytic bacteria isolates were isolated from the sterilized
Dendrobium
stems, and were classified into 43 species according to the 16S rRNA gene sequence analysis. Moreover, 14 of the 43 strains showed antimicrobial activity against phytopathogen using the Kirby-Bauer method. Strain NA-HTong-7 (
Bacillus megaterium
, 99.12%) showed the highest antimicrobial activity. This study was the first comprehensive study on endophytic bacteria of
Dendrobium
from different origins and cultivars, which provides new insights into the endophytic bacteria from
Dendrobium
.
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