Hairy enhancer of split-1 (HES1) is a transcriptional target of the Notch pathway, and a high level of HES1 is regarded as a marker of activated Notch. The aim of the study was to investigate the role of HES1 in colorectal cancer progression. We used tissue microarrays to analyze the expression and clinical significance of HES1 in 320 colorectal cancer samples. Stable overexpression and knockdown of HES1 were established in three colorectal cancer cell (CRC) lines (RKO, HCT8 and LOVO). We investigated the differentially expressed genes and enriched pathways in HES1 overexpressing CRC cells by gene expression profiling. Also, the role of HES1 in invasion and migration were examined in vitro and in vivo. We found that high expression of HES1 was significantly correlated with distal metastasis (P = 0.037) at diagnosis, and HES1 could serve as an unfavorable prognostic factor for colorectal cancer patients (P = 0.034). Gene expression profiling and pathway enrichment analysis revealed that HES1 was related to cellular adherens junction loss. In addition, we showed that HES1 overexpression lead to depressed E-cadherin, and elevated N-cadherin, vimentin and Twist-1 levels. Functionally, HES1 enhanced invasiveness and metastasis of CRC cells. HES1 promotes cancer metastasis via inducing epithelial mesenchymal transition and serves as a poor prognosis factor of colorectal cancer patients.
Fossilized charcoals can document how prehistoric humans used wood resources. Here, we present a quantitative result of charcoal fossil records, compiled with archaeobotanical data of Qijia Culture (4.3–3.8 cal. kyr BP) in the western Chinese Loess Plateau, to study how prehistoric people used plant resources and adapted to their environment. The charcoal records showed that Quercus, Ulmus, Pyrus, Acer, Picea, Prunus, and Bambusoideae were abundant during the Qijia Period, implying a warmer and wetter environment at that time. It also clearly showed that Qijia people had a strong preference to select woods in their daily life. Quercus was the most favorable wood, and thus, it was found in all samples. Many fruit trees planted in the Chinese Loess Plateau today were found in charcoal records. These fruit trees were, during the Qijia Period, at least in the process of being domesticated. Qijia people expanded the scope of using plant resources, and many new crops appeared during that time. These results provide a new insight into economic management and human adaption strategies in the Neolithic Age.
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