Anthocyanins, carotenoids, and betalains are known as the three major pigments in the plant kingdom. Anthocyanins are flavonoids derived from the phenylpropanoid pathway. They undergo acylation and glycosylation in the cytoplasm to produce anthocyanin derivatives and deposits in the cytoplasm. Anthocyanin biosynthesis is regulated by the MBW (comprised by R2R3-MYB, basic helix-loop-helix (bHLH) and WD40) complex. Carotenoids are fat-soluble terpenoids whose synthetic genes also are regulated by the MBW complex. As precursors for the synthesis of hormones and nutrients, carotenoids are not only synthesized in plants, but also synthesized in some fungi and bacteria, and play an important role in photosynthesis. Betalains are special water-soluble pigments that exist only in Caryophyllaceae plants. Compared to anthocyanins and carotenoids, the synthesis and regulation mechanism of betalains is simpler, starting from tyrosine, and is only regulated by MYB (myeloblastosis). Recently, a considerable amount of novel information has been gathered on the regulation of plant pigment biosynthesis, specifically with respect to aspects. In this review, we summarize the knowledge and current gaps in our understanding with a view of highlighting opportunities for the development of pigment-rich plants.
Past research has devoted more attention to the consequences of populism for party politics and governance than to the effects of populist claims on public attitudes—and particularly, to how populism interacts with nationalism to exacerbate exclusionary beliefs among the public. Using online survey experiments, we examine whether exposure to populism increases out-group antipathy among white Democrats and Republicans. In Study 1, we randomly assign respondents to three conditions featuring vignettes based on political speeches: a morally neutral argument, a populist critique of political elites, and a morally framed anti-immigration appeal. We then assess respondents’ sentiments toward Latinos, an ethnic group commonly vilified in U.S. right-wing political discourse. The results demonstrate that exposure to populist rhetoric alone results in lower feeling-thermometer ratings of Latinos, but only among Republicans. Study 2 uses a similar design to evaluate the link between left-wing economic populism and economic nationalism: the populist condition features a critique of economic elites, the nationalist condition blames China for the offshoring of U.S. jobs, and the outcome variable measures respondents’ sentiments toward China. Economic nationalism generates increased chauvinism among conservative Democrats but economic populism does not. Together these findings suggest that the cross-domain spillover effects between nationalism and populism observed in Study 1 are driven by the discursive bundling of anti-elite talk with ethno-nationalism on the right, which stands in contrast to the decoupling of economic populism and economic nationalism on the left. The former has effectively turned populism into a form of dog-whistle politics among Republicans.
Past research has devoted more attention to the consequences of populism for party politics and democratic governance than to its effects on public attitudes—and particularly, how populist claims interact with nationalism to exacerbate exclusionary beliefs among the public. Using online survey experiments, we examine whether exposure to populism increases out-group antipathy among Democrats and Republicans. In Study 1, we randomly assign respondents to three conditions featuring vignettes based on political speeches: a morally neutral argument, a populist critique of political elites and a morally framed anti-immigration appeal. The results demonstrate that the populist treatment generates lower feeling-thermometer ratings of minority groups than the control condition, but only among Republicans and Trump supporters. Study 2 uses a similar design to evaluate the link between left-wing economic populism and economic nationalism: the populist condition features a critique of economic elites, the nationalist condition blames China for the offshoring of US jobs and the outcome variable measures respondents’ sentiments toward China. Economic nationalism generates increased anti-China sentiment among moderate and conservative Democrats but economic populism does not. Together, these findings suggest that the effects of populism on nationalist antipathies observed in Study 1 are driven by the discursive bundling of anti-elite talk with ethno-nationalism on the political right, which stands in contrast to the decoupling of economic populism from economic nationalism on the left. The former has effectively turned populism into a form of dog-whistle politics among Republicans, and Trump supporters in particular.
Steroidal glycoalkaloids (SGAs) are cholesterol-derived molecules that contribute to the pathogen defense in tomato but are toxic and considered to be antinutritional compounds to humans. APETALA2/Ethylene Responsive Factor (AP2/ERF) family transcription factors (TFs) play an indispensable role in various biological processes, such as plant growth and development, fruit ripening, biotic and abiotic stresses responses, and SGA biosynthesis. In this study, we identified 176 AP2/ERF genes that were domesticated or improved SlAP2/ERF in the tomato variome (Solanum lycopersicum) within either domestication or improvement sweeps, respectively. According to the RNA-sequencing data, 93 of the ERF genes with high transcriptional level (Transcripts Per Million, TPM > 1) belong to six clusters. Weighted gene co-expression network analysis (WGCNA) and metabolite-based genome-wide association study (mGWAS) analyses revealed that the expression level of the Solyc04g071770 (SlERF.D6) gene in the cluster six gradually increased as the fruit matured. Transient transformation verified that the overexpression of SlERF.D6 significantly promoted fruit ripening and regulated the expression of multiple genes in the SGA synthesis pathway, thereby affecting the SGA content of the fruit. Virus-induced gene silencing (VIGS) showed that the silencing of SlERF.D6 delayed fruit ripening and influenced the content of SGAs. Our data provide new insights into AP2/ERF TFs in tomato, offer a candidate TF for fruit development and steroidal glycoalkaloids, and provide new resources for tomato breeding and improvement.
Congenital heart disease (CHD) with extracardiac malformations (EM) is the most common multiple malformation, resulting from the interaction between genetic abnormalities and environmental factors. Most studies have attributed the causes of CHD with EM to chromosomal abnormalities. However, multi‐system dysplasia is usually caused by both genetic mutations and epigenetic dysregulation. The epigenetic mechanisms underlying the pathogenesis of CHD with EM remain unclear. In this study, we investigated the mechanisms of imprinting alterations, including those of the Small nuclear ribonucleoprotein polypeptide N (SNRPN), PLAG1 like zinc finger 1 (ZAC1) and inositol polyphosphate‐5‐phosphatase F (INPP5F) genes, in the pathogenesis of CHD with EM. The methylation levels of SNRPN, ZAC1, and INPP5F genes were analysed by the MassARRAY platform in 24 children with CHD with EM and 20 healthy controls. The expression levels of these genes were detected by real‐time polymerase chain reaction (PCR). The correlation between methylation regulation and gene expression was confirmed using 5‐azacytidine (5‐Aza) treated cells. The methylation levels of SNRPN and ZAC1 genes were significantly increased in CHD with EM, while that of INPP5F was decreased. The methylation alterations of these genes were negatively correlated with expression. Risk analysis showed that abnormal hypermethylation of SNRPN and ZAC1 resulted in 5.545 and 7.438 times higher risks of CHD with EM, respectively, and the abnormal hypomethylation of INPP5F was 8.38 times higher than that of the control group. We concluded that abnormally high methylation levels of SNRPN and ZAC1 and decreased levels of INPP5F imply an increased risk of CHD with EM by altering their gene functions. This study provides evidence of imprinted regulation in the pathogenesis of multiple malformations.
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