This work describes the influence of coherent illumination on phase measurement accuracy in digital holographic microscopy (DHM). To improve net phase accuracy in a DHM system, the phase referencing and temporal averaging techniques are applied simultaneously to suppress the phase noises caused by the laser source and image sensor. A comparison between a laser diode operated in single- and multi-modes is given to demonstrate the coherence effect on the fringe visibility and the reconstructed phase accuracy of digital holograms. Axial sub-nanometer accuracy in DHM with the fewest successive hologram recordings is performed using a wavelength-stabilized laser diode in single-mode operation.
This work presents a close-packed synthetic aperture method for superresolution imaging in digital holographic microscopy. The superresolution imaging technique is based on beam-rotation approach to collect the different bandpass spectrum of the sample. The close-packed information from synthetic aperture process can be used to enhance the reconstructed imaging resolution under low-numerical-aperture microscope objective. Simulated and experimental results show the characteristics of the close-packed synthetic aperture and the influence on superresolution imaging.
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