No abstract

No abstract

No abstract

No abstract

No abstract

No abstract

Background The intestinal epithelial barrier, which works as the first line of defense between the luminal environment and the host, once destroyed, it will cause serious inflammation or other intestinal diseases. Tight junctions (TJs) play a vital role to maintain the integrity of the epithelial barrier. Lipopolysaccharide (LPS), one of the most important inflammatory factors will downregulate specific TJ proteins including Occludin and Claudin-1 and impair integrity of the epithelial barrier. Betaine has excellent anti-inflammatory activity but whether betaine has any effect on TJ proteins, particularly on LPS-induced dysfunction of epithelial barriers remains unknown. The purpose of this study is to explore the pharmacological effect of betaine on improving intestinal barrier function represented by TJ proteins. Intestinal porcine epithelial cells (IPEC-J2) were used as an in vitro model. Results The results demonstrated that betaine enhanced the expression of TJ proteins while LPS (1 μg/mL) downregulates the expression of these proteins. Furthermore, betaine attenuates LPS-induced decreases of TJ proteins both shown by Western blot (WB) and Reverse transcription-polymerase chain reaction (RT-PCR). The immunofluorescent images consistently revealed that LPS induced the disruption of TJ protein Claudin-1 and reduced its expression while betaine could reverse these alterations. Similar protective role of betaine on intestinal barrier function was observed by transepithelial electrical resistance (TEER) approach. Conclusion In conclusion, our research demonstrated that betaine attenuated LPS-induced downregulation of Occludin and Claudin-1 and restored the intestinal barrier function.

In this paper, a novel approach for controlling the direction of defect evolution in graphene through intercalation of organic small molecules into graphite oxide (GO) combined with a one-pot microwave-assisted reaction is reported. By using ethanol as intercalator, the bulk production of high quality graphene with its defects being satisfactorily healed is achieved. The repair of defects using extraneous carbon atoms and the hybrid state of these carbon atoms are definitely demonstrated using isotopic tracing studies with (13)C-labeled ethanol combined with (13)C solid-state NMR. The defect healed graphene shows excellent crystallinity, extremely low oxygen content (C : O ratio of 23.8) and has the highest sheet conductivity (61 500 S m(-1)) compared to all other reported graphene products derived from GO. By using methanol or benzene as intercalators, hierarchically porous graphene with a self-supported 3-dimensional framework (∼917 m(2) g(-1)) containing both macropores and mesopores (2-5 nm) is obtained. This graphene possesses a distinctive amorphous carbon structure around the edge of the nanopores, which could be conducive to enhancing the lithium storage performance (up to 580 mA h g(-1) after 300 cycles) when tested as an anode of lithium ion batteries, and might have promising applications in the field of electrode materials, catalysis, and separation, and so on. The mechanism involved for the controlled defect evolution is also proposed. The simple, ultrafast and unified strategy developed in this research provides a practical and effective approach to harness structural defects in graphene-based materials, which could also be expanded for designing and preparing other ordered carbon materials with specific structures.