Lipid peroxidation (LPO), an oxidative chain reaction often occurring in the human body and food, is harmful to human health. Due to its convenience and low cost, this guideline highlights the benefits and drawbacks of LPO models by spectrophotometer in vitro and in vivo. The preparation process and reaction mechanism of lipid substrates, oxidation triggers and detection methods are discussed in this paper to better screen antioxidants. The lipid substrates mostly consist of rat liver/brain homogenate, microsomal, low-density lipoprotein (LDL), and linoleic acid (LA), are appropriate for modeling oxidative damage in a variety of situations. Transition metals, ascorbic acid, 2,2′-azobis (2-amidinopropane) hydrochloride (AAPH), sodium Nitroprusside (SNP), 3-morpholinosydnonimine (SIN-1) and nicotinamide adenine dinucleotide phosphate (NADPH) make up the majority of the oxidizing triggers. The following LPO quantification techniques have been reviewed: (a) thiobarbituric acid reactive substances (TBARS) assay, (b) measurement of conjugated-diene (CV), (c) β-carrot bleaching assay, (d) thiocyanate method, (e) ferrous oxidationxylenol orange (FOX-2) assay. It can be concluded that more than one test system should be applied to obtain comprehensive results. In a word, this guideline provides a scientific basis for future research on antioxidants screening in food and body systems.
Anthocyanins, as a kind of natural pigment, have a broad development prospect in the field of human production and life due to its safety, nontoxic, rich resources, and rich pharmacological effects. However, due to the structure instability of anthocyanins, anthocyanins are susceptible to physical and chemical factors during food processing, such as light, temperature, pH, metal ions, food additives, oxidation reductants, and cochromatic factors. This review summarized the experimental methods for these factors affecting anthocyanin content, structural transformation, and degradation dynamics, which lays the foundation for further studies of anthocyanins in plants. Meanwhile, we expounded the methods for obtaining accurate quantitative and qualitative data of anthocyanin by UV-vis, HPLC, LC-MS, and NMR, which can provide a basis for further development and utilization of anthocyanin resources.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.