We report a 3D microfluidic pulsed laser-triggered fluorescence-activated cell sorter capable of sorting at a throughput of 23,000 cells sec−1 with 90% purity in high-purity mode and at a throughput of 45,000 cells sec−1 with 45% purity in enrichment mode in one stage and in a single channel. This performance is realized by exciting laser-induced cavitation bubbles in a 3D PDMS microfluidic channel to generate high-speed liquid jets that deflect detected fluorescent cells and particles focused by 3D sheath flows. The ultrafast switching mechanism (20 μsec complete on-off cycle), small liquid jet perturbation volume, and three-dimensional sheath flow focusing for accurate timing control of fast (1.5 m sec−1) passing cells and particles are three critical factors enabling high-purity sorting at high-throughput in this sorter.
Flexibility of energy consumption can be harnessed for the purposes of ancillary services in a large power grid.In prior work by the authors a randomized control architecture is introduced for individual loads for this purpose. In examples it is shown that the control architecture can be designed so that control of the loads is easy at the grid level: Tracking of a balancing authority reference signal is possible, while ensuring that the quality of service (QoS) for each load is acceptable on average. The analysis was based on a mean field limit (as the number of loads approaches infinity), combined with an LTI-system approximation of the aggregate nonlinear model. This paper examines in depth the issue of individual risk in these systems. The main contributions of the paper are of two kinds:Risk is modeled and quantified (i) The average performance is not an adequate measure of success. It is found empirically that a histogram of QoS is approximately Gaussian, and consequently each load will eventually receive poor service. (ii) The variance can be estimated from a refinement of the LTI model that includes a white-noise disturbance; variance is a function of the randomized policy, as well as the power spectral density of the reference signal. Additional local control can eliminate risk (iii) The histogram of QoS is truncated through this local control, so that strict bounds on service quality are guaranteed. (iv) This has insignificant impact on the grid-level performance, beyond a modest reduction in capacity of ancillary service.
During the last few decades microfluidic systems have become more and more popular and their relevance in different fields is continually growing. In fact, the use of microchannels allows a significant reduction of the required sample-volume and opens the way to a completely new set of possible investigations, including the study of the properties of cells, the development of new cells’ separation techniques and the analysis of single-cell proteins. One of the main differences between microscopic and macroscopic systems is obviously dictated by the need for suitable actuation mechanisms, which should allow precise control of microscopic fluid volumes and of micro-samples inside the fluid. Even if both syringe-pump and pneumatic-pump technologies significantly evolved and they currently enable sub-μL samples control, completely new approaches were recently developed for the manipulation of samples inside the microchannel. This review is dedicated to describing different kinds of optical actuators that can be applied in microfluidic systems for sample manipulation as well as for pumping. The basic principles underlying the optical actuation mechanisms will be described first, and then several experimental demonstrations will be reviewed and compared.
The Hancheng area is a hot spot for coalbed methane exploration and exploitation in China. Structure is a key factor affecting coalbed methane accumulation and production in the Hancheng area. For a better understanding of the coalbed methane accumulation conditions and high-yield potential, this study investigates the structural patterns and evolution, the hydrogeological conditions, and the geothermal field in the coal-bearing strata in the Hancheng area. Then, the spatial distribution of the coalbed methane content and the tectonic deformation of the coal seam are evaluated. Finally, the critical depth for coalbed methane enrichment and a high-yield
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