A set of hybrid rod-coil diblock copolymers containing different sizes of poly(3-hexylthiophene-2,5-diyl) (P3HT) as the rod block and a polystyryl-type (PS) coil block with covalently linked polyoxometalates (POMs) as side chains have been synthesized. The P3HT rod blocks, synthesized using Grignard metathesis polymerization, and the PS coil block, prepared by atom transfer radical polymerization were successfully coupled using "click" chemistry to form the rodcoil diblock copolymers (PS-PTn). POM cluster attachment was performed on the diblock copolymers through a post-
In order to better understand the mechanisms by which chondrocytes respond to mechanical stimulation, ATDC5 mouse embryonic carcinoma cells were induced to differentiate into chondrocytes and then exposed to mechanical loading. To specifically elucidate the role of this pathway, the localization and expression of proteins involved in the Wnt/β-catenin signaling pathway were observed. Chondrogenic-differentiated ATDC5 cells were exposed to a 12% cycle tension load for 1, 2, 4, or 8 h. At each time point, immunofluorescence staining, western blot analysis, and qPCR were used to track the localization of β-catenin and glycogen synthase kinase-3β (GSK-3β) expression. In addition, the mRNA expression of Wnt3a, disheveled homolog 1 (Dvl-1), GSK-3β, and collagen type II were also detected. Activation of the Wnt/β-catenin signaling pathway was investigated in cells treated with Dickkopf-related protein 1 (DKK-1). β-catenin and GSK-3β protein expression increased initially and then decreased over the mechanical loading period, and the corresponding mRNA levels followed a similar trend. After application of the inhibitor DKK-1, Wnt/β‑catenin signaling was suppressed, and the mRNA expression of collagen II was also reduced. Thus, stimulation of chondrocytes with mechanical strain loading is associated with the translocation of active β-catenin from the cytoplasm to the nucleus.
This article attempts to investigate the impacts of bilateral trade on the environment by estimating the embodied carbon emissions between China and Germany over the period 1999–2018. The above impacts are broadly explored in the literature both under the framework of theoretical and empirical analysis. However, there exist fewer empirical studies exploring the nonlinear relationship between trade volumes and carbon emissions between a well-developed and emerging economies. By applying the multiregional input-output (MRIO) model, this article aims to reveal the impacts of trade on the environment in the case of China–Germany. Specifically, trade amounts between China and Germany rank high with a similarly increasing trend and both of them are large net exporting countries. However, China experienced much larger carbon emissions embodied in its exports to Germany. Despite potential concerns on the carbon leakage issue of China from Germany, we find that the bilateral trades fit an inverse U-shape in the embodied carbon emissions, which suggests that the trade between the two countries can finally reduce carbon intensity without obstructing economic development particularly in the long-term. This paper guides policy-makers to quantify the issue of CO2 transfer among bilateral trades in order to achieve the target of trading sustainability.
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