Objective In the present study, we aimed to explore lncRNA HOXD cluster antisense RNA 1 (HOXD‐AS1) expression in oral squamous cell carcinoma (OSCC) tissues, its biological roles, and the underlying potential mechanisms in OSCC progression. Materials and Methods HOXD‐AS1 expression in paired OSCC and non‐tumor tissues from 60 OSCC patients was determined by RT‐qPCR. The effects of HOXD‐AS1 and miR‐203a‐5p overexpression on expression of Annexin A4, a validated target of miR‐203a‐5p, were analyzed by RT‐qPCR and Western blot. The roles of HOXD‐AS1, miR‐203a‐5p, and Annexin A4 in the invasion and migration of OSCC cells were analyzed by Transwell assays. Results HOXD‐AS1 overexpression in OSCC predicted poor survival. HOXD‐AS1 was predicted to interact with miR‐203a‐5p, but its expression was not significantly correlated with miR‐203a‐5p. HOXD‐AS1 overexpression increased Annexin A4 expression, while miR‐203a‐5p overexpression decreased Annexin A4 expression in OSCC cells. Transwell assays showed that invasion and migration of OSCC cells were enhanced by HOXD‐AS1 and Annexin A4 overexpression but were reduced by miR‐203a‐5p overexpression. In addition, miR‐203a‐5p overexpression suppressed the role of HOXD‐AS1 in cell movement and Annexin A4 expression. Conclusions HOXD‐AS1 may upregulate Annexin A4 by sponging miR‐203a‐5p in OSCC to promote cancer cell invasion and migration.
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