Evaluating biomechanical properties of trabecular meshwork (TM) is of great significance for understanding the mechanism of aqueous humor circulation and its relationship to some eye diseases such as glaucoma; however, there is almost no relevant study due to the lack of clinical measurement tool. In this paper, an acoustic radiation force optical coherence elastography (ARF‐OCE) system is developed with the advantages of noninvasive detection, high resolution, high sensitivity, and high‐speed imaging, by which elastic modulus of the porcine and human TMs is accurately quantified. As the first OCE imaging of TM, our study demonstrates that ARF‐OCE may be an effective approach to advance the research of diseases related to aqueous humor circulation.
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