A new SARS animal model was established by inoculating SARS coronavirus (SARS-CoV) into rhesus macaques (Macaca mulatta) through the nasal cavity. Pathological pulmonary changes were successively detected on days 5-60 after virus inoculation. All eight animals showed a transient fever 2-3 days after inoculation. Immunological, molecular biological, and pathological studies support the establishment of this SARS animal model. Firstly, SARS-CoV-specific IgGs were detected in the sera of macaques from 11 to 60 days after inoculation. Secondly, SARS-CoV RNA could be detected in pharyngeal swab samples using nested RT-PCR in all infected animals from 5 days after virus inoculation. Finally, histopathological changes of interstitial pneumonia were found in the lungs during the 60 days after viral inoculation: these changes were less marked at later time points, indicating that an active healing process together with resolution of an acute inflammatory response was taking place in these animals. This animal model should provide insight into the mechanisms of SARS-CoV-related pulmonary disease and greatly facilitate the development of vaccines and therapeutics against SARS.

Hydrogen peroxide is a widely used and important chemical in industry. A two-electron electrochemical oxygen reduction reaction (2e– ORR) is a clean and on-site method for H2O2 production. Here, we report metal-free catalysts (mesoporous carbon hollow spheres, MCHS) for high-efficiency H2O2 production in neutral electrolytes (0.1 M PBS). The selectivity of H2O2 on MCHS catalysts is higher than 90% at a wide range of potentials (0.35–0.62 V), and it can reach 99.9% at a potential of 0.57 V. These catalysts show some of the best performances for H2O2 production in neutral electrolytes. It is preferable to develop H2O2 catalysts in a neutral environment, as the pH of the stabilizers used for H2O2 is also close to neutral. The outstanding activity of our catalyst comes from a combination of factors such as suitable porosity, the content of oxygen functional groups, and the types of different species of oxygen functional groups. First-principles simulations show that a catalyst with suitable mixed oxygen and COOH functional groups plays an important role in the catalytic formation of H2O2. The reported metal-free catalysts are promising catalysts for high-efficiency production of H2O2 in the future.

Electrochemical oxygen reduction offers an efficient and environmental-friendly route for hydrogen peroxide (H2O2) generation. This electrochemical process can produces H2O2 on-site under ambient conditions. High selectivity and activity two-electron oxygen...

Abstract:Leaf area is an important plant canopy structure parameter with important ecological significance. Light detection and ranging technology (LiDAR) with the application of a terrestrial laser scanner (TLS) is an appealing method for accurately estimating leaf area; however, the actual utility of this scanner depends largely on the efficacy of point cloud data (PCD) analysis. In this paper, we present a novel method for quantifying total leaf area within each tree canopy from PCD. Firstly, the shape, normal vector distribution and structure tensor of PCD features were combined with the semi-supervised support vector machine (SVM) method to separate various tree organs, i.e., branches and leaves. In addition, the moving least squares (MLS) method was adopted to remove ghost points caused by the shaking of leaves in the wind during the scanning process. Secondly, each target tree was scanned using two patterns, i.e., one scan and three scans around the canopy, to reduce the occlusion effect. Specific layer subdivision strategies according to the acquisition ranges of the scanners were designed to separate the canopy into several layers. Thirdly, 10% of the PCD was randomly chosen as an analytic dataset (ADS). For the ADS, an innovative triangulation algorithm with an assembly threshold was designed to transform these discrete scanning points into leaf surfaces and estimate the fractions of each foliage surface covered by the laser pulses. Then, a novel ratio of the point number to leaf area in each layer was defined and combined with the total number of scanned points to retrieve the total area of the leaves in the canopy. The quantified total leaf area of each tree was validated using laborious measurements with a LAI-2200 Plant Canopy Analyser and an LI-3000C Portable Area Meter. The results showed that the individual tree leaf area was accurately reproduced using our method from three registered scans, with a relative deviation of less than 10%. Nevertheless, estimations from only one scan resulted in a deviation of >25% in the retrieved individual tree leaf area due to the occlusion effect. Indeed, this study provides a novel connection between leaf area estimates and scanning sensor configuration and supplies an interesting method for estimating leaf area based on PCD.

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Abstract:Timber volume is an important ecological component in forested landscapes. The application of terrestrial laser scanning (TLS) to volume estimation has been widely accepted though few species have well-calibrated taper functions. This research uses TLS technology in poplar (Populusˆcanadensis Moench cv. 'I-72/58') to extract stem diameter at different tree heights and establish the relationship between point cloud data and stem curve, which constitutes the basis for volume estimation of single trees and the stand. Eight plots were established and scanned by TLS. Stem curve functions were then fitted after extraction of diameters at different height, and tree heights from the point cloud data. Lastly, six functions were evaluated by R 2 and RMSE. A modified Schumacher equation was the most suitable taper function. Volume estimates from the TLS-derived taper function were better than those derived using the stem-analysis data. Finally, regression analysis showed that predictions of stem size were similar when data were based on TLS versus stem analysis. Its high accuracy and efficiency indicates that TLS technology can play an important role in forest inventory assessment.

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