We cloned and sequenced a 2.35-kilobase EcoRI fragment of genomic DNA from a local freshwater fish (Perca fluviatilis) that strongly hybridized to probes derived from the murine influenza virus resistance gene Mx. The cloned fish DNA contained blocks of sequences related to Mx gene exons 3 to 8, which appeared to represent exons of a bona fide fish gene because they were separated by intron sequences flanked by consensus splice acceptor and donor sites. Injection of double-stranded RNA into the peritoneal cavity of trouts resulted in 5-to 10-fold elevated levels of two liver mRNAs of about 2.0 to 2.5 kilobases in length that hybridized to the cloned genomic DNA. [24]). The blot was washed at 50°C in 40 mM phosphate buffer (pH 7) containing 1% sodium dodecyl sulfate and exposed to X-ray film for 3 days. HinidIII-digested DNA from the phage lambda served as size markers.
3117on May 10, 2018 by guest
The interfacial effect on the dynamical properties of spin-dependent polarons in an organic magnetic/nonmagnetic cooligomer is investigated by using a tight-binding model coupled with a nonadiabatic dynamic method. It is found that the dynamical behaviors of spin-up and spin-down polarons are quite different at the interface. In the presence of an external electric field, polarons with a specific spin (up or down) can get trapped near the magnetic/nonmagnetic interface while the motion of polarons with an opposite spin is not affected, which leads to the phenomenon of polaron spin-filtering. Interestingly, when the electric field is reversely applied, only polarons with opposite spin can pass through the co-oligomer and spin rectification can be observed. The spin-filtering and rectification can be improved by increasing the strength of the spin correlation in the magnetic part of the co-oligomer and they can also be affected by the strength of the interfacial electron−lattice coupling and the electronic transfer integral. These findings are useful for the design of organic-based spin logic devices.
Abstract. To investigate the genotype-phenotype correlation in chinese familial and sporadic hypertrophic cardiomyopathy, specific exons of the myosin binding protein-c gene (MYBPC3) were screened in six families with hypertrophic cardiomyopathy (HCM; FHCM) and in 20 patients with sporadic HCM (SHCM) from the anhui Province region of china. The V896M mutation was detected for the first time in China in two families with FHCM. The mutation was not found in 100 healthy control subjects. No mutations of MYBPC3 were detected in any of the SHCM patients. In contrast to previous reports, the V896M mutation may be a disease-causing mutation in China, and exon 27 of MYBPC3 may be a mutational hotspot in FHCM patients. However, mutations of MYBPC3 were not prevalent among SHCM patients.
Within the framework of the Su-Schrieffer-Heeger model modified to include uniform disorder and an external electric field, the polaron transport process in conjugated polymer material is simulated using a nonadiabatic evolution method. It is found that the polaron dynamic mechanism is determined by both the electric field and the uniform disorder. The effect of uniform disorder is not obvious and almost negligible in most cases, except for the case of very weak electric field. Compared with the effects of Gaussian disorder, the film morphology with uniform disorder is conductible to the polaron transport.
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